Georgia Tech Research Corporation

Ronald William Rousseau

Andreas Sebastian Bommarius

Athanassios Sambanis

Matt Mcdonald

Colton Lagerman

Martha Anna Grover

Walter L Kimble

Grant Marshall

Disclosed herein are devices, systems, and methods of use thereof for magnetic separation. For example, disclosed herein is a device for separation of a mixture comprising a magnetic solid and a non-magnetic solid. The device comprises a separation chamber extending from a proximal end to a distal end; the proximal end defining an inlet; the distal end defining a first outlet and a second outlet. The separation chamber having a region that is configured to subject the fluid flowing through said region to a magnetic field to thereby separate the mixture into a first portion and a second portion; the first portion being enriched with the non-magnetic solid relative to the second separated portion; and the second portion being enriched with the magnetic solid relative to the first separated portion.

Devices, systems, and methods of use thereof for magnetic separation

The invention is directed to a method of highly purifying a polypeptide or other biological compounds from a contaminant by crystallization comprising contacting a mixture containing uncrystallized polypeptides and a contaminant with an exogenous nucleating agent, crystallizing the polypeptides, thereby forming at least one crystal of the polypeptide attached to the nucleating agent, the attached crystal being of a high purity, and at least one polypeptide crystal unattached to the nucleating agent, the unattached crystal being of a lower purity than the attached crystal, and separating the crystal attached to the nucleating agent from the crystal unattached to the nucleating agent. Also, the invention is directed to a method of purifying a polypeptide in a high purity and in a high yield from a contaminant by crystallization comprising contacting a mixture containing uncrystallized polypeptides and a contaminant with an exogenous nucleating agent that has a close lattice match to the polypeptide, crystallizing the polypeptides, thereby forming at least one crystal of the polypeptide attached to the nucleating agent, the attached crystal being of a high purity and produced in a high yield, and at least one crystal unattached to the nucleating agent, the unattached crystal being of a lower purity than the attached crystal, and separating the crystal attached to the nucleating agent from the crystal unattached to the nucleating agent. The invention is also directed to compositions comprising highly purified polypeptide or biological compound crystals and compositions of highly purified polypeptides or biological compounds.

Crystallization and purification of polypeptides

Growing community of inventors

Atlanta, GA, United States of America

Ronald William Rousseau