Leland Stanford Junior University

The General Hospital Corporation

Verinata Health, Inc.

Gpb Scientific, LLC

Albert Einstein College of Medicine

Collaborative Research, Inc.

Whitehead Institute for Biomedical Research

Other

University of California

The Brigham and Women's Hospital, Inc.

Beth Israel Deaconess Medical Center, Inc.

Affymetrix, Inc.

Stratagene California

Gpr Scientific, LLC

Ronald W Davis

Nader Pourmand

Mehmet Toner

Ravi Kapur

William R Jacobs, Jr

Yang Liu

Daniel D Shoemaker

Roland B Stoughton

Richard A Young

Michael Nicholas Mindrinos

Miloslav Karhanek

Barry R Bloom

Mostafa Ronaghi

Robert N Husson

Thomas Willis

Utkan Demirci

Maneesh Jain

Lars Steinmetz

Gerald R Fink

Joseph L Horecka

Shan X Wang

Mark M Davis

Simon Fredriksson

R Fabian Pease

Barb Ariel Cohen

Eugeni A Namsaraev

Ulrich Schlecht

George Karlin-Neumann

Murat Baday

Jen-i Mao

Alison Taunton-Rigby

Donald T Moir

Frederick M Ausubel

Hesaam Esfandyarpour

Roland Stoughton

AmirAli Talasaz

MacDonald S Morris

Peter Edward Vaillancourt

Janine Mok

Mehdi Javanmard

Richard S Stephens

Michael P Mittmann

Hanlee P Ji

Robert W Dutton

Daniel Davis Shoemaker

Billy Tsz Cheong Lau

Naside Gozde Durmus

Paul Hardenbol

Viktor Stolc

Sujatha Krishnakumar

David Botstein

Robert P St Onge

Savas Tasoglu

Ionita C Ghiran

Thiruppathiraja Chinnasamy

Stefanie S Jeffrey

Jaeyoung Yang

Christopher G Goff

Robert G Knowlton

Semih Calamak

Chunlin Wang

Alessandro Tocchio

Sasha F Levy

Ashley Ann Powell

Sue S Kalman

Huseyin Cumhur Tekin

Guri N Giaever

Arthur Myles

Baback Gharizadeh

Kee-Hyun Paik

Peidong Shen

Daphne Preuss

Wayne Mitchell

Roxana Jalili

Henrik H J Persson

Jochen Kumm

Lisa Diamond

AmirAli Hajhossein Talasaz

Kelly Jo Davis

Amir Ali Haj Hossein Talasaz

Curt Scharfe

Ionita Ghiran

Billy Tsz Lau

Henrik HJ Persson

Amirali Hajhossein Talasaz

Michael Mindrinos

Fabian R Pease

A heterogeneous population of cells are separated and collected according to a method. The heterogeneous population of cells in a paramagnetic medium are placed in a fluidic channel in which the fluidic channel comprises two or more outlets. The heterogeneous population of cells in the fluidic channel are separated based on differences in magnetic susceptibility and density of the heterogeneous population of cells. Fluid comprising the separated cells is withdrawn from the two or more outlets using variable flow rates by fluidic pumps at respective ones of the two or more outlets simultaneously to fractionalize the fluid comprising the separated cells across the two or more outlets by manipulation of the variable flow rates relative to one another.

Sorting biological and non-biological moieties using magnetic levitation

Methods and apparatus for direct detection of chemical reactions are provided. Electric charge perturbations of the local environment during enzyme-catalyzed reactions are sensed by an electrode system with an immobilized target molecule. The charge perturbation caused by the polymerase reaction can uniquely identify a DNA sequence. The polymerization process generates local perturbations of charge in the solution near the electrode surface and induces a charge in a polarazible gold electrode. This event is detected as a transient current by a voltage clamp amplifier. Detection of single nucleotides in a sequence can be determined by dispensing individual dNTPs to the electrode solution and detecting the charge perturbations. Alternatively, multiple bases can be determined at the same time using a mix of all dNTPs with subsequent analysis of the resulting signal. This technique may be adapted to other reaction determinations, such as enzymatic reactions, other electrode configurations, and other amplifying circuits.

Charge perturbation detection system for DNA and other molecules

Magnetic cell levitation and cell monitoring systems and methods are disclosed. A method for separating a heterogeneous population of cells is performed by placing a microcapillary channel containing the heterogeneous population of cells in a magnetically-responsive medium in the disclosed levitation system and separating the cells by balancing magnetic and corrected gravitational forces on the individual cells. A levitation system is also disclosed, having a microscope on which the microcapillary channel is placed and a set of two magnets between which the microcapillary channel is placed. Additionally, a method for monitoring cellular processes in real-time using the levitation system is disclosed.

System and method for cell levitation and monitoring

The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.

Rare cell analysis using sample splitting and DNA tags

Provided herein is a circular proximity ligation assay in which proximity-probes are employed as bridges to connect two free oligonucleotides via a dual ligation event, resulting in the formation of a circle. The circles are then quantified by, e.g., qPCR. The addition of an extra oligonucleotide is believed to enhance specificity by decreasing the probability of random background ligation events. In addition, circle formation may have selective advantages, as uncircularized DNA can be removed by a simple exonuclease treatment and it has streamlined the workflow by eliminating preamplification prior to qPCR.

Highly specific circular proximity ligation assay

A novel method for preparing sequence-verified oligonucleotides is disclosed. In particular, the invention relates to a simple, affordable, and scalable method that combines high-throughput mating of yeast clones, a unique selectable system for combining DNA sequences in yeast, and next-generation sequencing. This method allows sequence-verified oligonucleotides to be readily isolated from complex libraries.

Scalable method for isolation and sequence-verification of oligonucleotides from complex libraries

The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, i.e. aneuploidy. In addition, the present invention provides methods to determine when there are insufficient fetal cells for a determination and report a non-informative case. The present invention involves quantifying regions of genomic DNA from a mixed sample. More particularly the invention involves quantifying DNA polymorphisms from the mixed sample.

Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats

Systems and methods for levitating populations of moieties, cells, or other such units using one or more magnets in a microfluidic environment are provided. These systems and methods may be used to, for example, separate or sort heterogeneous populations of the units from one another, to assembly a multi-unit assembly during the levitating of the units, and to evaluate samples at the point of care in real-time. These systems and methods may also utilize a frame that enables an imaging device, such as a smartphone, to capture the units in real time as they are manipulated in the system.

A shower gripping accessory device is provided which may comprise an elongate cylindrical body having a plurality of terminal ends. The elongate cylindrical body may comprise an outer shell body and a cavity therein. The thickness value of the outer shell body may be substantially between one-quarter to one-half a radius value of the cavity. Further, a multilayered frictional surface substrate may be disposed over the outer shell body. The multilayered frictional surface substrate may comprise a flexible polymer base layer, one or more adhesive resin layers and a frictional material layer. The one or more adhesive resin layers may couple the frictional material layer to the flexible polymer base layer.

Shower gripping accessory device

Methods are provided to determine the entire genomic region of a particular HLA locus including both intron and exons. The resultant consensus sequences provides linkage information between different exons, and produces the unique sequence from each of the two genes from the individual sample being typed. The sequence information in intron regions along with the exon sequences provides an accurate HLA haplotype.

Haplotying of HLA loci with ultra-deep shotgun sequencing

Growing community of inventors

Palo Alto, CA, United States of America

Ronald W Davis