Biosearch, LLC

Beckman Instruments, Inc.

Ronald M Cook

Derek Hudson

Jang B Rampal

Peter J Coassin

Kenneth D Konrad

Inverse Linkage Oligonucleotides ('ILO') useful in enzymatic process are disclosed. Particularly preferred ILOs are amenable to enzymatic elongation from either, or most preferably, both termini. In a particularly preferred embodiment, each terminus of an ILO has an enzymatically functional 3' group. Accordingly, under appropriate conditions and in the presence of, e.g., dNTPs, enzyme, sample DNA, and ILO comprising a first region complementary to a first region of the sample DNA and a second region complementary to a second, different region of the sample DNA, exponential amplification of the sample DNA can be effectuated.

Inverse linkage oligonucleotides for chemical and enzymatic processes

Benzazoles, including benzatriazoles and benzimidazoles are employed in phospate triester oligonucleotide synthesis. The benzazoles form arylbenzazolide coupling agents, and phosphorodibenzazolides. Processes employing these reagents are also disclosed.

Benzazolides and their employment in phosphate ester oligonucleotide

Benzotriazoles are employed in phosphite triester oligonucleotide synthesis. The benzotriazoles also form phosphinedibenzazolides. Processes employing these reagents are also disclosed.

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San Rafael, CA, United States of America

Ronald M Cook