Biosearch Technologies, Inc.

Other

United States of America As Represented by the Secretary of the Air Force

Millipore Corporation

Bioresearch Technologies, Inc.

Ronald M Cook

Matt Lyttle

Daren Dick

Derek Hudson

Rand Dill

Ben Ayer

Johnathan Lloyd Kiel

Mark Reddington

Kary B Mullis

Jeevalatha Vivekananda

Douglas J Ng

Marc P Beal

Mary Katherine Johannson

Jordan Honig

Eliana Saxon Armstrong

Hans E Johansson

Mary Katherine Johansson

Arnie Baltzley

Ben Sowers

Darren Dick

The present invention provides nucleic acid probes comprising two quenchers of excited state energy, and methods of their use.

Dual quencher probes

The present invention provides quenchers of excited state energy, probes and other conjugates comprising these quenchers, and methods of their use.

Cosmic quenchers

The present invention provides a family of dark quenchers, termed Black Hole Quenchers ('BHQs'), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.

Dark quenchers for donor-acceptor energy transfer

The present invention provides a new class of solids supports for synthesis of modified oligomers of nucleic acids, and nucleic acid probes that have a format expediently synthesized on the new supports. Exemplary solid supports include at least one quencher bound through a linker to the solid support. Various exemplary embodiments include a moiety that stabilizes a duplex, triplex or higher order aggregation (e.g., hybridization) of nucleic acids of which the oligomer of the invention is a component. Other components of the solid support include moieties that stabilize aggregations of nucleic acids, e.g., intercalators, minor groove binding moieties, bases modified with a stabilizing moiety (e.g., alkynyl moieties, and fluoroalkyl moieties), and conformational stabilizing moieties, such as those described in commonly owned U.S. Patent Application Publication No. 2007/0059752.

Stabilized nucleic acid dark quencher-fluorophore probes

Methods and compositions for immediately immunizing an individual against any molecule or compound are provided. The present invention is directed to an immunity linker with at least two sites; (1) at least one first binding site that binds to an immune response component in an individual, and (2) at least one second binding site that binds specifically to a desired compound or molecule, the target. The second binding sites are preferably thiolated aptamers that have the benefit of increased stability, resistance to degradation and longer circulating half life. Methods of making and using pharmaceutical compositions including immunity linker molecules having a thiolated aptamer are also provided.

Chemically programmable immunity

The present invention provides luminescent metal ion complexes for use in a wide range of biological and chemical studies. The luminescent metal ion complexes of the invention comprise a metal ion chelating component covalently bound to a carrier molecule. Also provided are methods of making and using the luminescent metal ion complexes.

Luminescent metal ion complexes

In one aspect, the invention provides fluorescent probes and assays. The probes include a fluorophore-quencher pair that undergoes a switch from dark to fluorescent in response to a reaction of the quencher. The switch of the probe from dark to fluorescent is typically mediated by an enzyme that acts directly or indirectly on the quencher, interfering with its ability to quench fluorescence emission from the fluorophore. In another aspect, the invention provides a reporter gene assay system and methods of using this system. The assay system includes a fluorophore-quencher probe and an enzyme that acts directly or indirectly on the quencher, increasing the fluorescent emission of the fluorophore. In still other aspects, the invention provides nucleic acid constructs and cells expressing the peptide products of these constructs. In assays of the invention, the presence of a target substance is detected by the switching of fluorescence mediated by the change in oxidation state of the quencher. The assay systems are of use in numerous assay formats, e.g., confirming the expression of the enzyme that acts on the quencher, and/or detecting a species conjugated to the enzyme.

Inducible fluorescence assay

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Novato, CA, United States of America

Ronald M Cook