University of California

Lawrence Livermore National Security, LLC

Ronald Li

Deborah K Lieu

Stephen M Lane

Michelle Khine

James W Chan

Thomas R Huser

Jing Liu

John C Rutledge

Chung-wah Siu

Douglas S Taylor

Theodore Zwerdling

Raman spectra of cells, such as normal human T- and B-cells from peripheral blood or human tonsil and the corresponding transformed cells are obtained by optically trapping the cells and obtaining their Raman spectra. The trapped cells can be subjected to one, two, or more different excitation wavelengths, and each wavelength of the corresponding Raman spectra can be stored in a separate channel. In preferred embodiments, two spectra are subtracted from each other to give a difference spectrum and each channel is analyzed independently to characterize the trapped cell. Alternatively, the Raman spectrum can be subjected to Principal Component Analysis (PCA) in order to characterize the trapped cell. The trapped cell thus classified can be sorted, or further manipulated.

Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components

Provided are devices and methods of preparing a population of cardiomyocytes by aligning undifferentiated pluripotent cells on a nanosacale textured surface.

Engineered physical alignment of stem cell-derived cardiomyocytes

Methods to prepare electrophysiology mature cells from immature cells are provided, without the need for genetic manipulation.

Electrophysiologically mature cardiomyocytes and methods for making same

This invention provides an isolated stem cell that has been modified to provide, enhance or contain the functional characteristics of the sarcoplasmic reticulum (SR). The isolated stem cells are modified in one or more of the following manners: by expressing a calcium channel protein; by expressing a calcium pump protein such as the sarcro/endoplasmic reticulum Ca2+-ATPase (SERCA) protein; by inhibiting or downregulating expression of the Na+/Ca+ exchanger (NCX) protein; by expressing a calcium handling protein; by expressing a transverse (t-tubule; and/or by expressing a transverse (t-tubule biogenic protein. After the cell has been modified, it may be expanded to a substantially homogenous population of these cells or alternatively, differentiated to a more mature cell type. Compositions containing these cells and population of cells are also provided by this invention. The cells and compositions can he used to regenerate cardiac tissue, improve cardiac function, restore action potential of cardiac tissue; and treat or prevent cardiac malfunction. These methods can be achieved by administering an effective amount of a cell or population of cells or tissue of this invention to a host in need thereof. The cells and population of cells can be used diagnostically to screen drug or other therapeutic candidates.

Compositions and methods for improving the functional efficacy of stem cell-derived cardiomyocytes

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Davis, CA, United States of America

Ronald Li