Bio-rad Laboratories, Inc.

Bio-rad Europe Gmbh

Ronald Lebofsky

Jeremy Agresti

Kalyan Handique

Nicholas Heredia

Jennifer Chew

George Karlin-Neumann

Man Cheng

Andrew Kohlway

Vishal Sharma

Samantha Cooper

Lucas Frenz

Sean Cater

Josh Shinoff

Christophe Quiring

Sophie Pierre

Rebecca Dievart

Nick Heredia

Zachary Burkett

Eric Pomaranski

Emilie Celine Claudie Macke

Rob Ray

Adam Mccoy

George Karlin-neumann

Inventions covered include methods, systems, and compositions for sample processing, involving morphology-adjustable (e.g., tunable on-demand) functionalized particles. In some embodiments, a method can include distributing a set of functionalized particles, in a first morphological state, across a set of partitions; transitioning the set of functionalized particles, at the set of partitions, from the first morphological state to a second morphological state; transitioning the set of functionalized particles, at the set of partitions, from the second morphological state to a third morphological state, and inducing interactions between the set of functionalized particles and a set of targets, within the set of partitions and according to a set of operations with a set of process fluids.

Compositions, methods, and systems for sample processing with morphology-adjustable functionalized particles

Methods and compositions are described for single cell resolution, quantitative proteomic analysis using high throughput sequencing.

Digital protein quantification

Methods and compositions are provided herein for preparing high-throughput cDNA sequencing libraries.

Second strand direct

Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.

Multiple beads per droplet resolution

Methods and systems for sample target molecules are provided.

Apparatus and method for fast digital detection

Improved multiple displacement amplification (MDA) reagents and methods are provided.

MDA using bead oligonucleotide

Methods are provided of assessing whether multiple differently-barcoded primers occur within the same partition.

Methods and compositions for deconvoluting partition barcodes

Methods and compositions for attaching cell-specific barcodes without formation of partitions is provided.

Cell barcoding for single cell sequencing

A system, method, and platform for target detection, the system including: a base substrate; a set of sample processing regions defined at a broad surface of the substrate, wherein each of the set of sample processing regions includes: a set of microwell subarrays arranged in a gradient between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions; and a cover substrate configured to mate with the base substrate in a coupled mode, the cover substrate comprising a network of venting channels aligned with the set of sample processing regions upon mating the base substrate with the cover substrate in the coupled mode, the network of venting channels providing gas exchange between the base substrate and an environment surrounding the microwell assembly. The invention(s) can be used for MPN assays.

System and method for target detection with applications in characterizing food quality and improving food safety

The invention(s) cover systems and methods for target detection in a multiplexed and rapid manner. Embodiments of the system can include: a base substrate; and an array of sample processing regions defined at a broad surface of the base substrate, wherein each of the array of sample processing regions includes: a set of microwell subarrays arranged in a gradient by volumetric capacity between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions. The system can support methods, with example implementation by an automated platform, for returning preliminary results from a subset of microwells of the samples processing regions, as well as results pertaining to specific and non-specific amplification, for multiple targets of a sample.

System and method for rapid multiplexed sample processing with applications for nucleic acid amplification assays

Methods and compositions are provided for making and using uniquely tagged target nucleic acid molecules.

Molecular barcoding

The methods and reagents are provided for barcoding and analysis of DNA samples using partition (e.g., droplet) technology while avoiding performing amplification in droplets.

Growing community of inventors

Kensington, CA, United States of America

Ronald Lebofsky