Cargill Inc.

Ronald J Evans

Steffen Schaffer

Hans H Liao

Eileen Spindler

Wendy Kathleen Ribble

Tanya Lipscomb

Liv Reinecke

Katrin Grammann

Jan Wolter

Catherine B Poor

Brittany Robinson

Brittany Prather

David A Hogsett

Dave A Hogsett

Brittany L Prather

Tanya Warnecke Lipscomb

Eileen C Spindler

A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.

Production of fatty acids esters

Growing community of inventors

Louisville, CO, United States of America

Ronald J Evans