Abvitro LLC

Ronald Hause

Francois Vigneault

Adrian Wrangham Briggs

Stephen Jacob Goldfless

Yue Jiang

Rajagopal Chari

François Vigneault

Provided herein are methods for target gene sequencing and single cell barcoding in conjunction with analysis of gene expression in single cells. In some embodiments, the target gene is an immune molecule, such as an antibody or TCR. In some embodiments, the methods can be used to carry out transcriptome sequencing, e.g., RNA sequencing, to capture transcriptome of single cells paired with full receptor immune receptor sequences such that information about the immune repertoire and transcriptome of a cell can be determined. Also provided are polynucleotide libraries for use in carrying out transcriptome analysis and immune molecule, e.g., antibody or TCR, sequencing.

Growing community of inventors

Seattle, WA, United States of America

Ronald Hause