Crucell Holland B.v.

Ronald H P Brus

Ton Logtenberg

Abraham Bout

Govert Johan Schouten

Alphonsus G C M UytdeHaag

Patrick H C Van Berkel

The invention provides methods for producing mixtures of antibodies from a single host cell clone, wherein, a nucleic acid sequence encoding a light chain and nucleic acid sequences encoding different heavy chains are expressed in a recombinant host cell. The recombinantly produced antibodies in the mixtures according to the invention suitably comprise identical light chains paired to different heavy chains capable of pairing to the light chain, thereby forming functional antigen-binding domains. Mixtures of the recombinantly produced antibodies are also provided by the invention. Such mixtures can be used in a variety of fields.

Recombinant production of mixtures of antibodies

The present invention provides novel methods for determining whether a compound influences a phase in the life cycle of a virus comprising providing a cell with the compound and with at least a fragment of the virus sufficient for performing the phase and determining whether the phase is influenced in the cell, the cell comprising a nucleic acid encoding an adenovirus early protein or a functional part, derivative and/or analogue of the adenovirus early protein. In another aspect, the invention provides the use of a cell, the cell comprising nucleic acid encoding an adenovirus early protein, for screening a library of compounds for the presence of a compound capable of influencing a phase in the life cycle of a virus capable of entering the cell. The invention also provides novel methods for identifying a compound with antiviral activity comprising providing a cell with at least a fragment of a virus, the fragment capable of performing a step in the life cycle of the virus, providing the cell with a compound and determining whether the compound is capable of influencing the step in the life cycle of the virus, wherein the cell comprises a nucleic acid encoding an adenovirus early protein or a functional part, derivative and/or analogue of the adenovirus early protein.

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Voorschoten, Netherlands

Ronald H P Brus