Gesellschaft Fur Biotechnologische Forschung Mbh (gbf)

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Bayer Aktiengesellschaft

Gesellschaft Fur Biotechnologische Forschung Gmbh

Gesellschaft Fuer Biotechnologische Forschung Mbh

Gsellschaft Fur Biotechnologische Forschung Mbh (gbf)

Gesellschaft Fur Biotechnologische Forschung Mbh Igbf)

Helmholtz-zentrum Fur Infektionsforschung Gmbh

Ronald Frank

Helmut Blocker

Sinan Guler

Hans Fritz

Wolfgang Bruns

John Collins

Werner Tegge

Andreas Meyerhans

Guido Volckaert

Wiebke Heikens

Stefen Hoffmann

Stefan Hoffmann

Friedhelm Maywald

Birgit Blankemeyer-Menge

Gudrun Heisterberg-Moutsis

Gisela Kurth

Andre Rosenthal

Stefan Matysiak

Olaf Schreuer

Heinrich Gausepohl

Ulrike Beutling

Krzysztof Bialek

Andrzej Swistowski

The present invention refers to a method for attaching substrate molecules to gel matrices, characterized in that a substrate is covalently bound to a monomeric or oligomeric constitutional unit of a gel and the resultant product is polymerized. The present invention further refers to gels obtainable by this process.

Immobilized substrate, separation gels and method for detecting enzymatic activity

The disclosed synthesis system is based on the idea of designing a synthesis and treatment procedure, substrates and anchor groups which enable biomolecules to be simultaneously produced in an entirely automatic manner. By using a pipetting robot to dispense the reagents, the reaction column can be arranged in a format suitable for subsequent treatment. For a pipetting robot to carry out even water-sensitive or air-sensitive synthesis protocols, certain structural measures must be taken. The operation principle of the automated and the synthesis sequence are described below as an example of a possible solution. The automation can work with conventional substrates and reagents. Handling, however, is simplified by new, specially adapted substrates and anchor groups. A special, simultaneous purification and aliquot portioning process improves product quality and makes the device easier to use.

Automated chemical synthesis apparatus

Process for synthesis of peptides

Protective or anchor groups and their use

A single, unitary, solid phase support apparatus having a planar surface divided into a plurality of separate zone functions to detect antibodies. Each zone has bonded to it, a different peptide through its C-terminal end. The zones are incubated with the analyte sample and observed for reaction, indicating the virus-specific or bacteria specific presence or absence.

Apparatus and methods for detecting antibodies

Peptide intermediates

The invention relates to a process for mild esterification of a sensitive carboxylic acid, in particular a suitably protected amino acid, with an alcohol component, the esterification according to the invention being carried out in the presence of 2,4,6-mesitylene-1-sulfonyl-3-nitro-1,2,4-triazolide (MSNT) and a weak base in an aprotic and non-basic solvent.

Process for mild esterification of a carboxylic acid with an alcohol

The present invention relates to a peptide having essentially the amino acid sequence of pancreatic secretory trypsin inhibitor (PSTI). The present invention also relates to variants of such peptide wherein one or more of the amino acids in the original sequence are replaced by other amino acids. These peptides show an advantageously modified specificity in their inhibotory action. A method of preparation of the peptides and their pharmaceutical use is also described.

Pancreatic secretory tryspin inhibitor and variants thereof produced by

A process for the purfication of synthetic oligonucleotides is described which drastically simplifies the hitherto very time consuming purification process. The deprotection solution is adsorbed without being concentrated on a carrier having reversed phase properties and optionally ion exchanger properties, the oligonuleotides without lipophilic protecting group are removed, their protecting group is cleaved off from the adsorbed oligonucleotides, the adsorbed oligonucleotides are washed and eluted from the carrier and then purified in a manner known per se.

Improved process for the purfication of synthetic oligonucleotides

The invention concerns a double-stranded recombinant DNA cloning vector comprising one single insertion site for foreign DNA and one or two labelling sites adjacent or approximate to said insertion site. In the instance of two labelling sites, said sites flank the insertion site. The labelling site(s) can be individually labelled in a fashion that results in the labelling of the 3' end of either strand. The use of the invention in a process affords the ability to individually label only one strand at the 3' end in a simplified method for base sequencing analysis.

Double-stranded vector and process using it

The present invention relates to a process for the simultaneous synthesis of several oligonucleotides on a solid phase by using supports of flat material. Finally, the present invention relates to an apparatus for carrying out said process.

Process for the simultaneous synthesis of several oligonucleotides on a

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Braunschweig, Germany

Ronald Frank