Unilever Patent Holdings B.v.

Ronald F De Winter

Paul James Davis

Martine Elisa Verhoeyen

Mark J Berry

Improved affinity purification media are provided by the use of small specific binding agents, especially Fv antibody fragments or single domain antibody fragments, immobilised on porous carriers having pore sizes in the range 30-1000 angstroms, preferably 30-300 angstroms. Silica is a preferred carrier. The small fragments are able to penetrate the pores and maximise the effective surface area of the carrier, and the microporous silica is sufficiently robust to be used at high pressure, so enabling the speed and/or throughput of a purification procedure to be increased.

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Bedford, United Kingdom

Ronald F De Winter