Topometrix Corporation

California Institute of Technology

Incyte Pharmaceuticals, Inc.

Nanostream, Inc.

Vestar, Inc.

Vestar Research, Inc.

Other

Advanced Telesensors, Inc.

Xerox Corporation

Nexstar Pharmaceuticals, Inc.

City of Hope National Medical Center

Ronald C Gamble

John D Baldeschwieler

Thomas P Theriault

Paul E West

Stephen D O'Connor

Lawrence Randolph Weill

Richard T Proffitt

Marc R Schuman

Jill P Adler-Moore

Paul G Schmidt

Steve Perry Monacos

George W Tin

Marcia R Mauk

Tsung-Ying Shen

Mitree M Ponpipom

Steven E Hobbs

Gary Fujii

Matthew M Gregori

Lawrence E Williams

Michael G Taylor

Paul G West

Scott C Winder

Albert M Lin

Sergey I Osechinskiy

Micheal C Hair

Sava R Lukac

Bruce Wilcox

A method for remotely sensing cardiac-related data of an animal subject includes transmitting a RF signal to impinge on tissue of the subject, receiving a reflected portion of the RF signal, generating baseband data, filtering baseband data (e.g., including high pass filtering), performing waveform phase position determination, performing at least one auto-correlation of the waveform phase position determined data, determining periodicity of the auto-correlated data, and (i) computing heart rate using a maximum periodicity of the periodicity, or (ii) identifying abnormalities in cardiac function, such as may be indicated by temporal variations in heart rate and/or signal amplitude corresponding to cardiac activity. Multiple bandpass filtering schemes may be employed.

System and method for facilitating reflectometric detection of physiologic activity

A parallel fluid processing system including multiple fluid process regions containing solid material in fluid communication with a common first fluid source may be used to conduct analyses and/or synthesis in parallel. A parallel fluid processing data correction method includes supplying and processing a calibrant in each fluid process region, measuring a first physical parameter and deriving at least one correction factor based on the parameter, supplying and processing at least one second fluid in each fluid process region, and then applying the correction factor to yield corrected process data. Retention time correction, peak area correction, and other useful data corrections may be performed. Parallel fluid processing may be performed with microfluidic devices and systems. A system for correcting retention times in parallel liquid chromatography is further provided.

Parallel fluid processing systems and methods

High throughput analytical systems and methods employing multiple liquid phase separation process regions coupled to a common mass spectrometer are provided. Disclosed systems and methods permits parallel separation and parallel storage of discrete eluate fractions, followed by sequential discharge and ionization of previously stored eluate portions to yield a composite ion stream containing the sequential series of eluate portions, followed by mass analysis of the ion stream. A common manifold may receive ions and utilize pressurized gas or ion gating to direct ions within the manifold toward the mass spectrometer inlet.

High throughput multi-dimensional sample analysis

Systems for analyzing multiple samples in parallel using mass spectrometric preferably coupled with fluid phase separation techniques are provided. A multi-analyzer mass spectrometer includes multiple inlets, multiple mass analyzers, and multiple transducers to conduct mass analyses of multiple samples in parallel. A modular mass analyzer may include a vacuum enclosure, a chassis, and multiple mass analysis modules disposed within the chassis. Modules are preferably disposed in a spatially compact two-dimensional array. A common multi-stage vacuum system may be utilized in conjunction with baffles or partitions disposed within and between modules to maintain differential vacuum conditions within the spectrometer utilizing a minimum number of pumps. Common control inputs may be provided to multiple modules or other components within a multi-analyzer spectrometer. Fluid phase separation devices for use with a multi-analyzer spectrometer may be microfluidic devices utilizing chromatographic, electrophoretic, or other separation methods.

High throughput systems and methods for parallel sample analysis

A method and apparatus are provided for preparing a substrate upon which is located microdrop-sized loci at which chemical compounds are synthesized or diagnostic tests are conducted. The loci are formed by applying microdrops from a dispenser from which a microdrop is pulse fed onto the surface of the substrate.

Method and substrate for performing multiple sequential reactions on a

Devices and methods are provided for precise reduction of arrays of microspots. A pulse jetting device is employed having a capillary of micron dimensions, with a portion of the capillary proximal to the jetting orifice circumferentially surrounded by a piezoelectric transducer. By appropriate design of the capillary, orifice and piezoelectric transducer, droplets can be formed on a surface, separated by as little as 80 microns center-to-center, and having at least about a 15 micron spacing at the border. The subject substrate arrays can be used for providing miniaturized arrays of reagents, such as nucleic acids, for detecting the presence of homologous sequences in a sample.

Jet droplet device

An apparatus for the automated synthesis of molecular arrays. A jetting device is employed along with a reaction chamber to dispense reagents used in the synthesis onto the substrate. A positioning system moves the substrate from the jet to the reaction chamber. A controller controls the movement of the substrate and the application of the reagents so that the synthesis is carried out according to a pre-determined procedure. The apparatus will synthesize nucleotides in an array of micron-size spots according to a pattern selected by the operator immediately prior to synthesis.

Apparatus for the chemical synthesis of molecular arrays

Devices and methods are provided for precise production of arrays of microspots. A pulse jetting device is employed having a capillary of micron dimensions, with a portion of the capillary proximal to the jetting orifice circumferentially surrounded by a piezoelectric transducer. By appropriate design of the capillary, orifice and piezoelectric transducer, droplets can be formed on a surface, separated by as little as 80 microns center-to-center, and having at least about a 15 micron spacing at the border. The subject substrate arrays can be used for providing miniaturized arrays of reagents, such as nucleic acids, for detecting the presence of homologous sequences in a sample.

A scanning force microscope is disclosed which includes integrated optics for viewing the optical lever arm, probe and sample to be examined. The scanning force microscope includes a simplified mounting of laser and related adjustments and the locating of a detector independent of the scanner, to improve ease of handling and providing convenient locations for adjustments. In one preferred embodiment of the scanning force microscope, the surface of samples may be imaged while the cantilever portion of the scanner is immersed in liquids without special set-up or special adapters.

Scanning force microscope with integral laser-scanner-cantilever and

Solvent vehicles and methods of their use in the pulse jet delivery of reagents in automated oligonucleotide synthesis by the solid-phase phosphoramidite method are provided. For pulse jet delivery of the activated monomer, acetonitrile in combination with at least one stabilizer co-solvent is employed as the solvent vehicle, where the stabilizer co-solvent is an inert, organic solvent having a boiling point of at least 100.degree. C. For pulse jet delivery of the detritylation reagent, a mono- or disubstituted methane is employed as the solvent vehicle, where substituents have a molecular weight greater than 36 daltons. Use of the solvent vehicles according to the subject invention provides for reduced reagent precipitation in the pulse jet tubes and improved results.

Methods and solvent vehicles for reagent delivery in oligonucleotide

Formulations consisting of phospholipid small unilamellar particles encapsulating polyene antifungal antibiotics and methods for using such compositions to treat systemic fungal infections are described. In a preferred embodiment, the particles are in the form of vesicles which comprise a polyene antifungal antibiotic, preferably amphotericin B and/or nystatin, egg phosphatidylcholine and cholesterol, preferably in the molar ratio of about 0.2 (AMB):2(PL):1(CHOL). These vesicles may also have an amine modified surface. The vesicles are suspended in a low isonic strength saccharide/tris solution at a pH of from about 6.0 to about 8.0 and may be administered to deliver the antifungal antibiotic to treat systemic fungal infections.

Treatment of systemic fungal infections with phospholipid particles

Methods for performing multiple sequential reactions on a matrix

A scanning probe microscope is disclosed which includes a pivotally mounted scanning assembly for scanning a surface of a sample. A sensing assembly is mounted relative to the scanning assembly and is positioned for sensing the surface topography of the sample. The scanning assembly includes a bore extending vertically through the scanning assembly which allows direct overhead viewing of the sensing assembly and the sample. The scanning assembly is pivotally mounted by a hollow pivot assembly which permits both the sensing assembly and the sample to be viewed through the scanning assembly when aligning the sensing assembly with the sample. The hollow pivot assembly also allows a user to optically view the sample from directly overhead with an optical microscope or charge coupled device.

Scanning probe microscope with hollow pivot assembly

The resonance contact scanning force microscope includes a reflective cantilever arm which is oscillated at a high harmonic of the resonance frequency of the cantilever arm, while the probe tip is maintained in substantially constant contact with the surface of the specimen. The motion of the free end of the cantilever arm is measured, to generate a deflection signal indicative of the amount of actual deflection of the probe tip. The method and apparatus permit high speed scans and real time imaging of the surface of a specimen with a substantial reduction in noise normally arising due to tip-surface interaction and acoustic noise.

Growing community of inventors

Altadena, CA, United States of America

Ronald C Gamble