Genentech, Inc.

Other

Washington Research Foundation

Genotypes, Inc.

Ronald A Hitzeman

Benjamin D Hall

Gustav Ammerer

George E Chisholm, Iv

Robert Tienhan Chang

David R Olsen

Franklin E Hagie Iv

Franklin E Hagie, Iv

George Chisholm

Hugh McMullin

Mark D Matteucci

Richard A Berg

David W Leung

Daniel G Yansura

Arthur D Levinson

Tina M Etcheverry

David P Toman

Gregory A Daniels

Chung N Chang

The present invention is directed to novel recombinant nucleic acids for introducing yeast chromosomal elements into the genomes of bacteria. The invention provides methods to convert the modified bacterial genomes into artificial yeast chromosomes by fusing the bacteria with yeast that linearize the modified bacterial genomes, to produce artificial chromosomes.

Automatic eukaryotic artificial chromosome vector

The invention discloses methods for effecting the production of recombinant mammalian procollagen in yeast, as well as compositions comprising yeast cells cap producing mammalian procollagen.

Expression of procollagen in yeast

Methods are disclosed for simplified recombinant production of fibrillar collagens. DNAs encoding fibrillar collagen monomers lacking the N propeptide, the C propeptide, or both propeptides are introduced into recombinant host cells and expressed. Trimeric collagen is recovered from the recombinant host cells.

Methods for the production of gelatin and full-length triple helical collagen in recombinant cells

Recombinant gelatin and full-length triple helical collagen

DNA expression vectors capable, in a transformant strain of yeast, of expressing a polypeptide under the control of a genetically distinct yeast promoter, processes of forming transformant strains of yeast and transformed yeast strains are disclosed.

Expression of polypeptides in yeast

A DNA expression vector capable, in a transformant strain of yeast, of expressing a biologically competent polypeptide ordinarily exogenous to yeast under the control of a genetically distinct yeast promoter, the polypeptide not being required for the growth of the transformant; the process of forming the transformant strain of yeast; and the transformant strain of yeast.

Described herein is the secretion from yeast of heterologous protein via a preprotein in which the heterologous protein is fused to the signal peptide of a yeast protein. The preprotein is processed by the cells to produce and secrete mature heterologous protein.

Use of yeast homologous signals to secrete heterologous proteins

Metallothionein transcription control sequences, promoters or inducing regions, free of DNA encoding metallothionein, are used to inducibly express genes encoding polypeptides of interest. Vectors and host expression systems using the transciption control sequences, promoters and/or inducing regions are provided.

Metallothionein transcription control sequences and use thereof

Hepatitis surface antigen is synthesized in recombinant yeast hosts transformed with vectors encoding hepatitis surface antigen, preferably under the control of the yeast PGK promoter and preferably in the absence of DNA encoding the surface antigen precursor. Hepatitis surface antigen is assembled by yeast into antigenic 22 nm particles even though hepatitis surface antigen bacterial transformants were not known to be capable of assembling the surface antigen into 22 nm particles.

Preparation of hepatitis b surface antigen in yeast

Yeast organisms are caused to express, process, and secrete protein that is normally heterologous to yeast and not required for its viability by transforming yeast with an expression vector containing a gene encoding heterologous protein and a signal sequence also heterologous to yeast.

Growing community of inventors

Pacifica, CA, United States of America

Ronald A Hitzeman