Ventana Medical Systems, Inc.

Osi Pharmaceuticals, Inc.

Ron Zeheb

Paula M Rodgers

John R Stephenson

Frederick H Reynolds, Jr

John M Sorvillo

Immunoassay for detection of mutant P53 polypeptide in serum

The present invention provides a liquid evaporation inhibitor which is suitable for use in high temperature applications; e.g., above 70.degree. C. The evaporation inhibitor liquid comprises a mixture of an alkane, a non-volatile oil, preferably a vegetable oil and a surfactant. The alkane is present in the mixture in an amount sufficient to provide a density which promotes floating on an aqueous medium and to cover an aqueous medium. The non-volatile oil is present in the mixture in an amount sufficient to inhibit evaporation of the mixture at temperatures above 70.degree. C. The surfactant is present in the mixture in an amount sufficient to maximize the contact surface area between the evaporation inhibitor liquid and the aqueous medium.

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Tucson, AZ, United States of America

Ron Zeheb