Biomerieux, Inc.

Biomerieux Vitek, Inc.

Ron Robinson

Craig Drager

Mark Joseph Fanning

William Ernest Seaton

Roger James Morris

Jean-Pierre Bernard Gayral

David B Shine

Clifford W Karl

Michael James Justin

Thomas Burchard

Paul Springer

JoAnne T Gerst

Brent D Freiner

Mark S Wilson

David Paul Chastain

James Clement Bishop

Andrew K Moore

Gregory R Maes

Erik W Anthon

David Porat

Donald L Meyer

Garry R Tegeler

Arthur Rousmaniere

Andrew G Zeigler

Richard James Ryall

Dennis M Connor

David Rosten

John Hoffman

Stephen Guerrera

Richard B Partlow

Brian Flynn

Daniel Ray Williams

Terrance J McMichael

Bernard Jean Limon

Raymond M Shelton

Bruno Colin

John D Walsh

Jones M Hyman

Christopher S Ronsick

John G Link

Sylvain Baeyaert

Gregg Flender

Chris Ronsick

John Walsh

Mark Wilson

The present invention is directed to a separation device or container that can be used in the separation, isolation or pelleting of microorganisms from a test samples known to contain or suspected of containing said microorganisms. Subsequently, the separated, isolated or pelleted microorganism sample can undergo one or more interrogation steps to provide measurements useful for the characterization and/or identification of microorganism. In one aspect of the present invention, the interrogation steps can occur in situ in the separation device or container described herein.

Separation device for use in the separation, characterization and/or identification of microorganisms

Stopper

An incubation station has a carousel for receiving a plurality of test sample cards. An even temperature and air flow distribution inside the carousel and incubation of the cards over time at the proper temperature was found to be critically dependent upon air flow characteristics within the incubation station. To optimize air flow, the carousel was given an open rear side that is exposed to an air distribution table having a cover plate placed adjacent to the rear surface of the carousel. The cover plate has a plurality of elongate slots positioned in a symmetrical ring-shaped pattern in registry with the carousel. The elongate slots are positioned at an angle relative to the slots in the carousel such that each carousel slot receives air from more than one slot. An extra fan was also added to the system to increase the flow of air into the air table. Scallop features on the carousel walls also promote even distribution of air flow over the cards. The carousel may be divided into a plurality of carousel segments to increase ease of removal and insertion of the carousel into the incubation station and manufacturability of the carousel.

Incubation station for test sample cards

Carousel for incubation station

An automatic sample testing machine for testing samples stored in test cards. The machine has a test sample positioning system for moving a tray containing a plurality of test sample cards and fluid receptacles among various stations in the machine. The machine has a diluting station for adding a predetermined quantity of diluent to the receptacles as needed. A pipetting station transfers fluid from one receptacle to another. A vacuum station is provided having a vacuum chamber moveable relative to the tray between upper and lower positions. The chamber cooperates with the tray to make a sealing engagement with the top surface of the tray when it is lowered to the lower position. A vacuum generator supplies vacuum to the chamber. When the vacuum is released from the chamber, the fluid samples are loaded into the cards from the receptacles. The test sample positioning system moves the tray to a cutting and sealing station and then to an incubation station and loads the cards one at a time into a carousel within the incubation station. A test card transport station transports the test cards from the incubation station to an optical reading station, where optical measurements (e.g., transmittance and/or fluorescence optical testing) is conducted on the wells of the card. When the card has been read, it is either moved back to the incubation station for additional incubation and reading or transferred to a card disposal system when the reading is complete.

Automatic sample testing machine

Test sample cards containing biological samples are moved through an analytical instrument past a card detection device and detected by the device. The card detection device has an actuator that reciprocates relative to a housing between first and second positions. The actuator has a surface that comes into contact with the card as the card is moved past the device. The actuator carries an optical interrupt flag which is used in conjunction with an optical sensor placed in proximity to the actuator. When the card comes into contact with the actuator, the actuator is moved from the first position to the second, or retracted position. This action moves the flag relative to the optical sensor, triggering the optical sensor and a detection of the card. In one possible embodiment, multiple cards are placed in a cassette for movement around the instrument. The cassette has a number of slots for the cards, one card per slot. Each card has placed thereon indicia such as bar codes that are used for identification purposes by the instrument. The card detection device, in addition to performing the detection functions for each of the cards, also rocks the cards within the cassette slots so as to place the bar codes or other indicia into a better position for reading by a bar code reader or other appropriate reading device for the indicia.

A sample card transport station moves a test sample card from an incubation station for the card to a transmittance and fluorescence optical station in a sample testing machine. The sample card transport station has a drive belt and an associated stepper motor. The belt supports the card from one side of the card. A ledge having a card slot is disposed above the belt. The card is snugly received within the card slot, and supported from below by the drive belt and rollers for the belt. When the motor turns the belt, the belt grips the card and slides the card along the slot to the optical stations, without any slippage between the belt and the card. This construction provides for precise control over the movement of the card. The fluorescence station has a linear flash lamp that illuminates a column of the wells of the cards simultaneously. A reference detector and dichromatic beam splitter design is used to insure that the fluorescence measurements are independent of lamp output changes over time. A solid reference is mounted to an optical shuttle and moved into the optical path to calibrate the fluorescence photodiode detectors.

Reflector assembly for fluorescence detection system

A sample card transport station moves a test sample card from an incubation station for the card to a transmittance and fluorescence optical station in a sample testing machine. The sample card transport station has a drive belt and an associated stepper motor. The belt supports the card from one side of the card. A ledge having a card slot is disposed above the belt. The card is snugly received within the card slot, and supported from below by the drive belt and rollers for the belt. When the motor turns the belt, the belt grips the card and slide the card along the slot to the optical stations, without any slippage between the belt and the card. This construction provides for precise control over the movement of the card. The fluorescence station has a linear flash lamp that illuminates a column of the wells of the cards simultaneously. A reference detector and dichromatic beam splitter design is used to insure that the fluorescence measurements are independent of lamp output changes over time. A solid reference is mounted to an optical shuttle and moved into the optical path to calibrate the fluorescence photodiode detectors.

Fluorescence station for biological sample testing machine

Cutting and sealing station for test sample cards in an automated

Optical reader and sample card transport stations for biological sample

Identification system for test sample cards in an analytical instrument

Automatic sample testing method

A positioning system for a sample testing machine incudes a base pan and a set of independent drive systems for sliding a sample tray across the base pan. Each of the drive systems incudes a paddle that engages a side of the sample tray. The paddle is slideably mounted to a shaft with a collar that slides over the shaft. A motor and drive belt is provided to move the collar along the shaft, causing the paddle to engage the side of the sample tray and slide the sample tray across the base pan. Track sections are provided in the base pan to prevent the sample tray from rotating as the paddle slides the sample tray across the base pan.

Test sample positioning system

Closure for a box for straws

A positioning system for a sample testing machine includes a base pan and a set of independent drive systems for sliding a sample tray across the base pan. Each of the drive systems includes a paddle that engages a side of the sample tray. The paddle is slideably mounted to a shaft with a collar that slides over the shaft. A motor and drive belt is provided to move the collar along the shaft, causing the paddle to engage the side of the sample tray and slide the sample tray across the base pan. Track sections are provided in the base pan to prevent the sample tray from rotating as the paddle slides the sample tray across the base pan.

Growing community of inventors

Bridgeton, MO, United States of America

Ron Robinson