Merck Patent Gmbh

Romas Skudas

Mikhail Kozlov

Matthew T Stone

Kevin Galipeau

Bianca Edelmann

Wilson Moya

Michael Schulte

Matthias Joehnck

Klaus Adrian

Sven Andrecht

Bianca Edlemann

Simon Braun

Lars Peeck

Oliver Rammo

Annika Holzgreve

Alisa Strobel

Dominic Zorn

The present disclosure relates to methods of protein purification by attaching an intein-C fragment to a target protein, passing a sample containing the intein-C tagged protein over a chromatographic resin carrying an intein-N fragment so as to create an intein-N intein-C complex, releasing the target protein from the intein-C fragment, and regenerating the column under conditions that disrupt the intein-N intein-C complex while preserving column functionality for multiple reuses.

Reuse of intein-bound resins for protein purification

The present invention relates to a method for the separation and purification of glycoforms with an ion exchange separation material with amino-acid based endgroups.

Glycoform purification

A method and an apparatus suitable for a continuous chromatography process which only needs three separation columns, and a two-step process containing two chromatographic steps, in which the first chromatographic step (capture) is performed alternating and sequentially on two separation columns, the second chromatographic step (polishing) is performed, also sequentially, on the third column.

Method and apparatus for chromatographic purification

The present invention relates to the purification of target molecules like recombinant and/or biotherapeutic proteins. Activated carbon can be used to remove leachables and/or extractables resulting from disposable equipment employed in the process.

Activated carbon for the removal of leachables and/or extractables

The present invention refers to a method for the separation of peptide aggregates and fragments from solutions containing target peptide.

Target molecule capture from crude solutions

The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal, of protein, fragments without adversely affecting the yield of the desired protein product.

Removal of fragments from a sample containing a target protein using activated carbon

The present invention refers to a method for the separation of host cell proteins (HCPs), antibody fragments and low molecular weight substances from solutions containing antibodies.

Robust antibody purification

The present invention is directed to a continuous affinity chromatography method and to an apparatus to be used in such method. The method allows the use of high operational velocity while maintaining high binding capacities.

Growing community of inventors

Mainz, Germany

Romas Skudas