Solvay Enzymes Gmbh & Co. Kg

Genencor International, Inc.

Kali-chemie Aktiengesellschaft

Gesellschaft Fuer Biotechnologische Forschung Mbh

Gesellschaft Fuer Bio-technologische Forschung

Roman Vetter

Detlef Wilke

Antoine Amory

Ingo Muecke

Bernhard Moeller

Pierre Ledoux

Gerhard Konieczny-Janda

Wolfgang Aehle

Eric De Buyl

Andre Clippe

Dietmar Schomburg

Andr&eacute;e Lahaye

Christophe Andre

Ren&eacute; Detroz

Meike Takenberg

Martina Mueller

Amory Antoine

Harald Sobek

Birgit Foullois

A purified xylanase derived from PRL B12 is disclosed. This xylanase is efficient for use in the biobleaching of wood pulp, permitting a strong reduction in the quantity of chlorine used and AOX compounds produced in classical and ECF wood pulp bleaching sequences as well as the quantity of ozone used in TCF sequences. The gene coding for the xylanase was isolated and purified and used to construct an expression vector therefor. A recombinant host strain of is also disclosed which is efficient for expressing heterologous enzymes, including the xylanase when transformed by the expression vector.

Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof

Xylanase derived from a bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof

Alkaline Bacillus proteases, their use and a method for producing these proteases are described. These are in particular Bacillus proteases from Bacillus pumilus DSM 5777. The alkaline proteases according to the invention are suitable for use in compositions for cleaning and washing purposes.

Alkaline proteases from Bacillus pumilus

Enzymes stabilized against the destabilizing effect of ionic surfactants and a method of producing such stabilized enzymes in which DNA sequences which code for the enzymes are modified by directed mutation in defined positions which correlate with defined surface regions of the enzyme, in such a way that the codon in which the mutation is located now codes for an amino acid differing from the original amino acid.

Stabilized enzymes and process for preparing them

Alkaline bacillus lipases, coding DNA sequences therefor and bacilli,

Novel, optimized highly alkaline proteases which are suitable for use in detergent formulations are prepared by employing microorganisms transformed by mutated DNA sequences. The mutated sequences are obtained starting from DNA sequences which code for highly alkaline protease usually produced by Bacillus species by altering these DNA sequences in defined positions by directed mutagenesis (point mutation) in such a way that the codon in which the point mutation is located now codes for an amino acid which is more strongly basic than the original amino acid. The result is highly alkaline proteases in which original amino acids have been replaced by more strongly basic amino acids, preferably by the amino acids lysine or arginine. Synthetic oligonucleotides, DNA sequences, vectors and transformed microorganisms which are used for generating and obtaining the optimized highly alkaline protease are also described.

Highly alkaline proteases

Growing community of inventors

Burgdorf, Germany

Roman Vetter