Alliance for Sustainable Energy, LLC

Alliance for Sustainably Enegry, LLC

Roman Brunecky

Michael E Himmel

Stephen Robert Decker

Jeffrey G Linger

John Aikens

Venkataramanan Subramanian

Yannick J Bomble

Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays.

Enzyme scaffolds and methods of use

Disclosed herein are methods of making multifunctional microbial cellulases. The engineered multifunctional microbial cellulases disclosed herein exhibit improved activity over native cellulases.

Multifunctional microbial cellulases

Disclosed herein are enzymes and combinations of the enzymes useful for the hydrolysis of cellulose and the conversion of biomass. Methods of degrading cellulose and biomass using enzymes and cocktails of enzymes are also disclosed.

Enzymes for improved biomass conversion

Growing community of inventors

Arvada, CO, United States of America

Roman Brunecky