Carl Zeiss Microscopy Gmbh

Carl Zeiss Microimaging Gmbh

Carl Zeiss Jena Gmbh

Cad Zeiss Microscopy Gmbh

Rolf Wartmann

Joerg Sprenger

Lars Stoppe

Hubert Wahl

Michael Brehm

Harald Schadwinkel

Jan Thirase

Benedict Diederich

Kerstin Winkler

An objective for a microscope includes a displaceable lens group for correcting a spherical aberration. The displaceable lens group is designed in so that an offset of same in the direction perpendicular to the optical axis leads to only a small coma.

Objective for a microscope

An object transfer function for a sample object is determined on the basis of a reference measurement. Subsequently, an optimization is carried out in order to find an optimized illumination geometry on the basis of the object transfer function and an optical transfer function for an optical unit.

Structured illumination with optimized illumination geometry

The invention relates to a system for spherically correcting imaging of a three-dimensional object, comprising a microscope comprising an immersion medium and an embedding medium for holding the three-dimensional object separated from the immersion medium of the microscope by a boundary surface or a cover slip. According to the invention, the thickness (&#x394;s) of the immersion medium and the position (&#x394;s') of the primary intermediate image of the three-dimensional object can be variably modified.

Microscope for the spherically corrected imaging of three-dimensional objects

An apochromatic microscope objective, including three optical sub-systems, wherein starting from the object plane, the first sub-system includes a meniscus and a convergent lens, wherein the meniscus is curved towards the object plane, the second sub-system is made up of three elements, wherein a first element includes a meniscus or a cemented element, a second element is configured as a collecting cemented element and a third element is a cemented element, and wherein either the first element or the third element is strongly scattering and the third sub-system has at least one cemented element with a collecting lens.

Apochromatic microscope objective

The invention relates to a high aperture immersion objective particularly for uses in confocal microscopes where oil is the immersion fluid, which objective is composed of three lenses and/or subsystems comprising lens groups. An apochromatic correction in a range from 365 to 900 nm is achieved at high resolving numeric apertures of 1.3 to 1.4 and an object field from 0.4 to 0.625 mm by the specification of the optical components. Additionally, the immersion objective has sufficiently good transparency up to a wavelength of 340 nm.

High aperture immersion objective

An optical system for digital microscopy having at least one lens, at least one telescope of the Kepler type and tube optics, wherein an aperture is arranged between the telescope and the tube optics, which aperture is imaged by means of the telescope at the rear focal point of the lens and the following conditions are met simultaneously:

Optical system for digital microscopy

A device for three-dimensional measurement of an object, with a stereomicroscope of the telescope type, which has a common objective and a first and a second tube lens, a first image sensor, which is assigned to the first tube lens, a second image sensor (), which is assigned to the second tube lens, and a control unit, which determines the three-dimensional shape of the object from the images obtained with the two image sensors and carries out a predetermined three-dimensional measurement, wherein the stereomicroscope is designed such that the images are obtained with object-side telecentric conditions of the stereomicroscope.

Device and method for the three-dimensional measurement of an object

The invention relates to an optical inspecting system designed to image an object to be inspected with a variable imaging scale, comprising a variation system for imaging the object into infinity, a lens group downstream of the variation system for imaging the object from infinity into the image plane of the whole system, and a light source for generating light in order to illuminate the object, means for imaging the illuminating light into the exit pupil of the variation system being provided in the airspace between the fifth lens group and the subsequent lens group. Of the five lens groups of the variation system, the first, second, and fourth lens groups, when seen in the imaging direction, are arranged in a movable manner in the direction of the optical axis, whereas the third and fifth lens groups are not movable.

Optical inspection system with a variation system consisting of five lens groups for imaging an object into infinity

A high-aperture immersion objective, in particular for confocal applications in fluorescence microscopy and for TIRF applications, having three subsystems of lenses and/or lens groups. The design of the subsystems has made it possible for a relatively large object field of 0.25 mm to be present in the case of a high-resolution numerical aperture of 1.49. Furthermore, improved transparency is possible up to a wavelength of 340 nm.

High-aperture immersion objective

A tube unit for microscopes which has a tube lens, including two components with an intermediate, large air separation, and an overall positive refractive power. The air separation is at least half the size of the focal length f of the tube lens. A roof edge mirror or another suitable deflection element is arranged between the two components of the tube lens. The roof edge mirror includes two mirrors, which can be tilted with respect to one another, and which is able to be tilted around its roof edge. The tilting movement or the tilting angle of the tiltable mirror or deflection element corresponds to half the tilt or half the tilting angle of the tube or eyepiece viewing system.

Tube unit for microscopes

An optical system for arranging between a light source and a field diaphragm in an illumination beam path of a microscope comprises n imaging optical elements with focal lengths fand Abbe numbers &#x3bd;(i=1, . . . , n). The following relationship is met for the optical system: where his one half of the bundle diameter of a bundle of light rays proceeding from a point of the light source at the entrance to the imaging optical element i.

Optical system

The invention is directed to a high-aperture optical imaging system, particularly for microscopes, which comprises an objective and a tube lens unit and in which the objective has a magnification of less than or equal to 40&#xd7; and a numerical aperture of greater than or equal to 1.0 and is chromatically corrected up to the infrared.

High-aperture optical imaging system, particularly for microscopes

The invention is directed to a stereoscopic microscope objective. A microscope objective of this kind contains five lenses. The first lens, considered from the object side, has a focal length between &#x2212;2.11f and &#x2212;1.20f; f is the total focal length of the microscope objective. This is followed by a second lens with a focal length between 1.30f and 1.45f, a third lens with a focal length between 1.15f and 1.37f, a fourth lens with a focal length between 0.65f and 1.7f, and a fifth lens with a focal length between &#x2212;0.96f and 0.48f. Combined, the amount of the refractive power of the last two lenses is less than 0.31f. In the first lens and fifth lens, at least 80% of the refractive power is concentrated on the image-side surfaces. In the second lens, at least 60% of the refractive power is concentrated on the image-side surface. In the third lens, at least 60% of the refractive power is concentrated on the object-side surface. In the fourth lens, at least 70% of the refractive power is concentrated on the image-side surface. The first lens and the fifth lens are made of a material having an anomalous partial dispersion of a first type. The second lens, third lens and fourth lens are made of a material having an anomalous partial dispersion of a second type.

Stereoscopic microscope objective

The invention is directed to an immersion microscope objective. This microscope objective contains four lens groups. The first lens group has a focal length of 2.6f&#x2013;3.1f, where f is the total focal length of the microscope objective. The first lens group contains two lens components with a collecting effect. The first lens component has a focal length of 3f&#x2013;4.5f. One of the lenses in the first lens component is made from a material with n greater than 1.72. A lens of the second lens component is made from a material with a partial dispersion of a first type. The second lens group with a focal length of 8f&#x2013;10.2f comprises at least two lenses which are cemented together. A collecting lens is made from a material with a partial dispersion of the first type, and a diverging lens is made from a material with a partial dispersion of a second type. There follows a third lens group with a focal length greater than |25f|. The third lens group-times contains at least two lenses which are cemented together, one of which is a collecting lens made from a material with a fluor crown anomaly. Finally, there follows a fourth lens group with a focal length greater than |6&#x2154;f| and comprising two meniscus-shaped lenses whose hollow surfaces face one another.

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Waake, Germany

Rolf Wartmann