Henkel Kommanditgesellschaft Auf Aktien

Henkel Kommanditgesellschaft Auf Aktien (henkel Kgaa)

Henkel Research Corporation

Rolf Schmid

Joachim Schindler

Wolfgang Preuss

Michael Bahn

Albrecht Weiss

Alfred Struve

Frank F Hill

Friedhelm Bartnik

Rudiger Wagner

Ludwig Schieferstein

Adolf Asbeck

Christian G Paech

Werner Stein

Charles R Wilson

Konrad Engelskirchen

Juergen Plapper

Robert B Reynolds

Hans F Pfeiffer

Dieter Hansen

Karlheinz Maurer

Nicholas C Kennedy

Sherman S Hom

Jonathan R Mielenz

Beth F Ladin

Martina Markgraf

A proteolytic enzyme for use in detergent formulations having increased pH and oxidative stability under typical laundering conditions in aqueous solutions is produced by fermenting Bacillus licheniformis host strain transformed by a multicopy plasmid comprised of DNA sequences which code for the desired proteolytic enzyme.

Alkaline proteolytic enzyme and method of production

A novel microorganism, mutant chol 149-K14 (DSM 2065) and process for the preparation of 3a.alpha.-H-4.alpha.-(3'-propionic acid)-5.alpha.-hydroxy-7a.beta.-methyl-hexahydro-1-indanone and the .delta.-lactone thereof comprising culturing a steroid compound of animal or plant origin with the above microorganism mutant in an aqueous nutrient medium under aerobic conditions.

Process and microorganism for degrading steroids

In the aerobic culture of microorganisms of the Xanthomonas genus in fermentation media, the use of water-in-oil emulsions in the media minimizes viscosity problems and enhances yields of Xanthomonas biopolymers. Preferably, the emulsion is formed with a surfactant and the microorganism is Xanthomonas campestris.

Process for producing Xanthomonas biopolymers

Process for the preparation of acid stable protease having a broad activity spectrum in high yields from mutants of the fungus Aspergillus, and the mutants used therein.

Acid stable protease from mutants of genus Aspergillus

Process for the preparation of acid stable protease having a broad activity spectrum by culturing selected mutants of Rhizopus rhizopodiformis CBS 227.75 under aerobic conditions in a nutrient medium containing assimilable carbon and nitrogen sources, at a pH between 3 and 7 and at a temperature between 25.degree. C. and 50.degree. C., and separating the resulting protease enzyme; the mutants used in the process together with a process for preparation of the mutants.

Acid stable protease from mutants of genus Rhizopus

The degradation of steroid-C22-carboxylic acids to the C20-carbonyl steroids is carried out in simplified form and preferably without isolating intermediate reaction products by using 17(20)-steroid-22-carboxylic acids as starting material and directly transforming them into the 20-carbonyl compound through their respective acid halides by the Curtius degradation or by carboxy-inversion degradation. The acetyl substituent thus formed in C17 of the steroid compounds is present in the pharmacologically required configuration.

Simplified process for the degradation of steroid-C-22-carboxylic acids

For the production of 17-C-steroid-.alpha.-propionic acid compounds by microbial side chain degradation on 17-C-side chain steroid substrates wild strain microorganisms selected in predetermined manner are subjected to a multi-stage mutation and selection. The resultant mutant strains are capable of supplying the desired compounds, e.g. .DELTA.-4 BNC and/or .DELTA.-1,4 BNC in high yields even in the absence of inhibitors arresting the growth and/or degradation of the steroid ring. Wild strains and mutants particularly suitable, and their choice, production and use, are described.

Process for the preparation of defective mutants of microorganisms

A .DELTA..sup.1,4,17 -BNC compound having the formula: ##STR1## wherein X is a substituent selected from the group consisting of OH and OCH.sub.3 ; as well as its process of production by microbiological side-chain splitting of a 17-C-side-chain steroid with a defect mutant microorganism which gives a steroid with a 17-C-.alpha.-propionic acid substituent in the absence of inhibitors inhibiting either the cleavage of the steroid ring or microorganism growth, where the defect mutant microorganisms are grown under a reduced air supply whereby an enrichment of .DELTA..sup.1,4,17 -BNC occurs.

3-Oxo-pregna-1,14,17-trien-20-carboxylates and process

A process for the production of useful steroids obtained by side chain degradation of sterol source materials through the use of microorganisms is described.

Steroid production

The present invention concerns a process for the softening of furs while at the same time taking the greatest possible care of the appearance of the hair. The process of this invention comprises contacting a fur with an acid aqueous liquor containing an acid protease from a fungus strain of the genus Rhizopus rhizopodiformis, said acid protease being effective in the pH range of from about 2.5 to 6.5.

Growing community of inventors

Dusseldorf, Germany

Rolf Schmid