Bayer Technology Services Gmbh

Unversität Zürich

Eidgenossische Technische Hochschule Zurich

Eidgenossisch Technische Hochschule Zurich

Rolf Hofer

Marcus Textor

Michael Pawlak

Markus Ehrat

Eveline Schürmann-Mader

Samuele Tosatti

Jeffrey Alan Hubbell

Nicholas David Spencer

Donald L Elbert

Laurence Ruiz-Taylor

Stephanie Finken

The invention relates to a method for precipitating mono or multiple layers of a mixture of organophosphoric acids of the general formula I (A)Y&#x2014;B&#x2014;OPOH&#x2003;&#x2003;(IA)or of organophosphonic acids of the general formula I (B)Y&#x2014;B&#x2014;POH&#x2003;&#x2003;(IB)and the salts thereof, wherein Y and B are defined in the specification. According to the invention, precipitation occurs on substrate surfaces of pure or mixed oxides, nitrides or carbides of metals and semiconductors, in particular as surfaces of sensor platforms, implants and medical accessory devices. The invention also relates to the use thereof as part of coated sensor platforms, implants and medical accessory devices.

Method for precipitating mono and multiple layers of organophosphoric and organophosphonic acids and the salts thereof in addition to use thereof

The invention relates to a method for precipitating mono or multiple layers of organophosphoric acids of the general formula I (A)Y&#x2014;B&#x2014;OPOH&#x2003;&#x2003;(IA)or of organophosphonic acids of the general formula I (B)Y&#x2014;B&#x2014;POH&#x2003;&#x2003;(IB)and the salts thereof, wherein B is an alkyl, alkenyl, alkinyl, aryl, aralkyl, hetaryl or hetarylalkyl residue and Y is hydrogen or a functional group from the hydroxy, carboxy, amino, optionally low-alkyl-substituted mono or dialkylamino series, thiol, or a negative acid group from the ester, phosphate, phosphonate, sulfate, sulfonate, maleimide, succinimydyl, epoxy, acrylate series, wherein a biological, biochemical or synthetic indicator element may be coupled to B or Y by addition or substitution reaction, wherein compounds may also be added conferring the substrate surface a resistance to protein adsorption and/or to cell adhesion and in the B chain may optionally be comprised one or more ethylene oxide groups, rather than one or more &#x2014;CH2&#x2014; groups. According to the invention, said precipitation occurs on substrate surfaces of pure or mixed oxides, nitrides or carbides of metals and semiconductors, comprising the use of water-soluble salts of a compound of formula (IA) or (IB) for the treatment of these surfaces, in particular as surfaces of sensor platforms, implants and medical accessory devices. The invention also relates to the use thereof as part of coated sensor platforms, implants and medical accessory devices in addition to new organophosphoric acids and organophosphonic acids themselves. The optionally substituted compounds of formulae (IA) and (IB) wherein the B and Y groups have the above-mentioned designations, i.e. optionally substituted alkyl, alkenyl, alkinyl, aryl, aralkyl, hetaryl or hetaryl, are hereinafter referred to as organophosphoric acids and organophosphonic acids.

The invention relates to a method for precipitating mono or multiple layers of organophosphoric acids of the general formula I (A)Y&#x2014;B&#x2014;OPOH&#x2003;&#x2003;(IA)or of organophosphonic acids of the general formula I (B)Y&#x2014;B&#x2014;POH&#x2003;&#x2003;(IB)and the salts thereof, wherein B is an alkyl, alkenyl, alkinyl, aryl, aralkyl, hetaryl or hetarylalkyl residue and Y is hydrogen or a functional group from the hydroxy, carboxy, amino, optionally low-alkyl-substituted mono or dialkylamino series, thiol, or a negative acid group from the ester, phosphate, phosphonate, sulfate, sulfonate, maleimide, succinimydyl, epoxy, acrylate series, wherein a biological, biochemical or synthetic indicator element may be coupled to B or Y by addition or substitution reaction, wherein compounds may also be added conferring the substrate surface a resistance to protein adsorption and/or to cell adhesion and in the B chain may optionally be comprised one or more ethylene oxide groups, rather than one or more &#x2014;CH2- groups. According to the invention, said precipitation occurs on substrate surfaces of pure or mixed oxides, nitrides or carbides of metals and semiconductors, comprising the use of water-soluble salts of a compound of formula (IA) or (IB) for the treatment of these surfaces, in particular as surfaces of sensor platforms, implants and medical accessory devices. The invention also relates to the use thereof as part of coated sensor platforms, implants and medical accessory devices in addition to new organophosphoric acids and organophosphonic acids themselves. The optionally substituted compounds of formulae (IA) and (IB) wherein the B and Y groups have the above-mentioned designations, i.e. optionally substituted alkyl, alkenyl, alkinyl, aryl, aralkyl, hetaryl or hetaryl, are hereinafter referred to as organophosphoric acids and organophosphonic acids.

Multifunctional, polyionic copolymers with molecular architectures and properties optimized for specific applications are synthesized on/or applied to substrate surfaces for analytical and sensing purposes. The coatings are particularly useful for suppression of non-specific interaction, adsorption or attachment of molecular or ionic components present in an analyte solution. Chemical, biochemical or biological groups that are able to recognize, interact with and bind specifically to target molecules in the material containing the analyte to be detected can be coupled to, integrated into, or absorbed to the multifunctional copolymers. These multifunctional copolymer coatings are compatible with a variety of different established methods to detect, sense and quantify the target molecule in an analyte. The multifunctional copolymer coatings typically include brush copolymers based on a polycationic or polyanionic (jointly referred to herein as 'polyionic') backbone with side chains that control interaction with the environment, such as poly(ethylene glycol) or poly(ethylene oxide)-based side chains that decrease cellular adhesion, and analyte-specific side chains. They can be used to pattern the surfaces into non-adhesive and specifically adhesive areas by applications of known techniques such as microfluidic or contact printing techniques.

Multifunctional polymeric surface coatings in analytic and sensor devices

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Dachsen, Switzerland

Rolf Hofer