Ge Healthcare Bio-sciences Ab

Ge Healthcare Bio-sciences Corp.

Cytiva Bioprocess R&d Ab

Ge Healthcare Bioprocess R&d Ab

Rolf Hjorth

Klaus Gebauer

James Van Alstine

Jamil Shanagar

David Ronnholm

Martin Hall

Karol Lacki

Thomas Falkman

Gunnar Malmquist

Patrick Jonsson

Magnus Asplund

Camilla Estmer Nilsson

Shujian Yi

Emmanuel Macedo

David Rönnholm

Hans O Johansson

A disposable container comprising a side wall, top and bottom, wherein the side wall comprises a first flexible material and the bottom comprises a rigid or semi-rigid material. The side wall top and bottom are joined together to define the container with an interior compartment for keeping a fluid inside the container. The bottom comprises at least one opening that is fluid-tightly covered with an assembly comprising a second flexible material. The assembly comprises at least one port which provides access to the interior compartment of the container. The invention also relates to a mixing system comprising the disposable container.

Disposable container and mixing system comprising the container

A generally cylindrical collapsible mixing vessel, comprising: a) a flexible plastic film side wall, a top wall and a bottom wall, wherein both the top wall and the bottom wall are attached to the side wall and the side, top and bottom walls define an inner volume of the vessel; b) an agitator located in the inner volume and; c) at least one vortex breaker made from flexible plastic film, located in the inner volume and attached to the bottom wall and/or the side wall via string elements, wherein said vortex breaker extends along the side wall and inwards in a generally radial direction.

Collapsible bag with flexible vortex breaker

A container comprising a first material and a second material, the first material being a flexible material and the second material being a rigid or semi-rigid material and at least partially defining an interior compartment for keeping a fluid inside the container characterized in that the flexible material and/or the semi-rigid or rigid material have thermoplastic properties and wherein the flexible material and the semi-rigid or rigid material are joined together by a seal or joint which comprises a heat seal and an adhesive seal.

Container with a seal and a method of making the seal

The present invention relates to a process of enriching one target compound from a liquid, which process comprises at least one step of isolation performed by differentially partitioning between two aqueous phases. In the present invention the phases are formed by adding a thermally responsive, self-associating (i.e. clouding) hydrophilic polymer, and if needed some additional salts, to an aqueous biotechnical solution (such as a fermentation sample or bioseparation process stream) under thermal and other conditions where the solution separates into a one polymer, two-phase system with one phase enriched in the polymer. The target compound is to be found in the phase not enriched in the polymer, while a significant though varying percentage of contaminants may differentially partition to the phase interface or the polymer enriched phase. With minor or no modification the target containing phase solution can be further processed via standard unit operations such as precipitation, chromatography, and filtration to further purify target and remove any residual polymer.

Separation method using single polymer phase systems

The present invention relates to a liquid mixture comprising a first polymer, which is a poly(acid), a second polymer, which is a poly(ether), and at least one salt, wherein the molecular weight of the poly(acid) is in the range of 1000-100,000 Da. The second polymer is selected to be capable of forming immiscible aqueous phases in the presence of the poly(acid) and salt. The poly(acid) may be selected from the group consisting of poly(acrylic acid) and poly(methacrylic acid), and the second synthetic polymer may comprise ethylene oxide. The invention may be used for separation of biomolecules, cells or particles.

Polymer two phase system and use thereof

A tank adapted to be used for suspending separation media slurry before transferring the separation media slurry to a column or a container comprises:

Separation media slurry tank

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Uppsala, Sweden

Rolf Hjorth