Centro De Inmunologia Molecular

Biotech Pharmaceutical Co. Ltd.

Rolando Pérez

Xianhong Bai

Loany Calvo

Kaiheng Xiao

Zhenhua He

Yuemao Liu

Miguel Arias

Xiao Chen

MeyLen Chea

Tamara González

Yangliu Cai

Zhenhua Yang

Julio Palacios

Zhi Bai

Provided is a method for obtaining high-yield, stable expression cell clones from myeloma cell lines in a protein-free culture medium. The method is used for industrial production of a recombinant antibody, and includes three stage: (1) adapting to a protein-free culture medium, statically culturing cells at a low density, and gradually reducing a fat-rich supplement to a chemical culture medium; (2) adapting to a protein-free culture medium; culturing cells at a high density, and using a perfusion fermentation system in a laboratory scale; and (3) screening high-yield, stable expression cell clones from the cells after fermentation ends. The cell clone may be used to produce a humanized anti-NeuGcGM3 14F7 recombinant antibody.

Method for obtaining high-yield, stable expression cell clones and antibody molecules obtained thereby

Growing community of inventors

Havana, Cuba

Rolando Pérez