Boehringer Mannheim Gmbh

Roland Schenk

Dietmar Zdunek

Arno Deger

Christian Klein

Erasmus Huber

Gerhard Bienhaus

Judith Pinsl-Ober

The present invention provides a process for the determination of a specifically bindable substance by incubation of the sample solution with at least three receptors R.sub.1, R.sub.2 and R.sub.3, of which R.sub.1 and R.sub.2 are bindable with one another and R.sub.3 is specifically bindable with the substance to be determined and measurement of the agglutination which takes place in the case of the reaction, wherein, as receptor R.sub.1, there is used a conjugate of a partner of a specifically binding pair P and of a substance S which corresponds to the substance to be determined or is a derivative thereof and has at least one epitope of the substance to be determined, as receptor R.sub.2 there is used a receptor which has at least two binding positions for P and as receptor R.sub.3 there is used a receptor which has at least two binding positions, of which at least one binds specifically with an epitope of the substance to be determined or of S. The present invention also provides a reagent for carrying out the above process.

Process and reagent for the determination of a specifically bindable

The application describes homogeneous assays for determining ligands. A sample is contacted with a labeled substance P.sub.2 and receptor R. The receptor R consists of one molecule of P.sub.1, which monovalently and specifically binds to P.sub.2, and one molecule for R.sub.1, which binds to the ligand of interest. Complexes of P.sub.1, P.sub.2, R.sub.1 and ligand form, and are determined. R.sub.1 must be a nucleic acid molecule.

Nucleic acid hybridization based assay for determining a substance of

Method for the immunological determination of a ligand in which the ligand to be determined is reacted with

Method for the immunological determination of ligands

For the determination of a specifically bindable substance by incubation of the sample solution with at least two receptors R.sub.1 and R.sub.2, whereby R.sub.1 and R.sub.2 are capable of binding to each other and R.sub.1 is capable of specific binding to the substance to be determined and measurement of the agglutination which occurs in the reaction, a conjugate of one partner of a specific binding pair P and a component K capable of specific binding to the substance to be determined is used as the receptor R.sub.1 and a receptor which has at least two binding sites for P is used as R.sub.2.

Process for the determination of a specifically bindable substance

The invention provides a cleaning solution and a method of using the cleaning solution for cleaning diagnostic analyzers. The solution contains a rapidly wetting surfactant and an inorganic or organic acid. The invention provides rapid and effective cleaning of proteins from all types of materials used for components of automatic analyzers.

Method for cleaning a diagnostic analyzer

Hapten-biotin conjugates in which the hapten is linked with biotin via a spacer, which has 26 to 40 atoms in its chain and contains at least 5 heteroatoms, are novel and are suitable, in particular for use in a competitive homogeneous immunoassay in which the agglutination which occurs in the reaction is evaluated by turbidimetric or nephelometric measurements.

Hapten-biotin conjugates and their use

The present invention provides a process for the determination of a specifically bindable substance by incubation of a sample solution with at least three receptors R.sub.1, R.sub.2 and R.sub.3, of which R.sub.1 is specifically bindable with R.sub.2 and R.sub.3, as well as with the substance to be determined, R.sub.2 brings about the binding to a solid phase and R.sub.3 carries a labelling, separation of bound labelling from unbound labelling and measurement of the labelling in one of the two phases, wherein, as receptor R.sub.1, a receptor is used which has at least two binding positions which bind specifically with an epitope of the substance to be determined, as R.sub.2 a conjugate of a partner P.sub.1 of a specifically binding pair and of a substance S which corresponds to the substance to be determined or is a derivative thereof and has at least one epitope of the substance to be determined, the partner P.sub.1 thereby either being bound to a solid phase or being immobilized, and as R.sub.3 a complex is used which contains at least the substance S and a labelling.

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Weilheim, Germany

Roland Schenk