Qiagen Gmbh

Qiagen Gaithersburg Inc.

Cube Biotech Gmbh

Roland Fabis

Bernd Springer

Christoph Erbacher

Christoph Ritt

Thomas Manz

Philippe Sauer

Ralf Himmelreich

Thomas Rothmann

Thorsten Voss

Mario Scherer

Markus Müller

Jörg Hucklenbroich

Thomas Hanselle

Jan Petzel

Ralf Peist

Dirk Loeffert

Sabine Werner

Kerstin Steinert

Joachim Ribbe

Helge Lubenow

Holger Wedler

Yuri Khripin

Thi My Chi Nguyen

Sabine Dorit Menzel

Andreas Sch&auml;fer

Anke Homann

Nadine Krueger

Melanie Emmerlich

Axel Deeg

Thorsten Träger

Anke Homann-Wischinski

Sabine Scheltinga

Sabine Jennrich

Markus MÜLLER

A solid-phase chelator material usable for the purification of proteins. The solid-phase chelator material comprises a solid phase, polyamine groups bound to the solid phase and chelating groups bound to the polyamine groups. At least a part of the polyamine groups is connected with at least two chelating groups per polyamine group. Each chelating group comprises one or several aminopolycarboxylic acid groups (APA groups), with the proviso that the number of APA groups per polyamine group connected with at least two cheating groups is at least three.

Solid-phase chelator material, method for producing thereof and use thereof for the purification of proteins

The present invention relates to a lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids and a method for isolating and/or purifying nucleic acids.

Lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids

The present invention relates to a chromatographic device for isolating and purifying nucleic acids, preferably genomic DNA, by gel filtration chromatography, a method for isolating and purifying nucleic acids, preferably genomic DNA, using this device and a kit comprising this device.

Chromatographic device and method for isolating and purifying nucleic acids

The present invention provides a cost-efficient method for producing particles having a SiOcontaining surface wherein said method comprises a) providing an aqueous reaction composition comprising i) core particles, ii) an added base, iii) a silicate salt, and iv) a pH modulator wherein the pH value of the reaction composition is above the gelation pH value; b) agitating said reaction composition, wherein the pH modulator decreases the pH value of the reaction composition over time and wherein due to said decrease of the pH value of the reaction composition SiOis deposited onto the core particles, whereby particles are formed which have a diameter of 30 &#x3bc;m or less; and c) obtaining the particles. Furthermore, silica particles having high nucleic acid binding properties are provided.

Preparation of silica particles

The present invention relates to a method for purifying nucleic acids from a sample containing nucleic acids, the method comprising at least the following steps: a. bringing the sample containing nucleic acids into contact with a nucleic acid binding phase comprising protonatable groups, wherein the protonatable groups have a pKs value of 9 to 12; b. binding the nucleic acids to the nucleic acid phase at a pH (binding pH) that is at least one pH unit less than the pKs value of at least one of the protonatable groups; c. eluting the nucleic acids at a pH greater than the binding pH but at least one pH unit less than the pKs value of at least one of the protonatable groups. Also disclosed are corresponding kits and nucleic acid binding phases that can be used for purifying nucleic acids.

Nucleic acid purification method

The invention relates to the preparation of a biological sample for performing verifications and examinations, wherein the aim of the invention is the creation of a method for preparing a biological sample having an improved PCR sensitivity compared to the reference standard having standard PCR without having to raise the cost thereof.

Preparation and amplification of nucleic acids by means of magnetic particles

The present disclosure relates to methods for isolating, amplifying, and/or analyzing nucleic acids in the presence of an anion exchange material by performing the isolation, amplification and/or analysis step in the presence of at least one anionic compound.

Compositions, methods, and kits for isolating and analyzing nucleic acids using an anion exchange material

The present invention relates to a method for isolating and purifying nucleic acids, preferably comprising genomic DNA, from biological samples comprising the steps of lysing the sample using a lysis buffer comprising a source of anionic surfactant ions, optionally disintegrating the RNA present in the lysate, precipitating the surfactant ions from the lysate, and separating the nucleic acids from the precipitate and further contaminants by size-exclusion chromatography. The invention furthermore relates to a lysis buffer, a method of lysing cells and a kit for the isolation and purification of nucleic acids.

Method for isolating and purifying nucleic acids

The present invention relates to a method for purifying nucleic acids using a nucleic acid-binding phase which is furnished with a deficit of nucleic acid-binding groups A having a pK of 8 to 13, or which has groups A and binding-inhibiting groups N which are neutrally charged during the binding, and preferably during the elution, and the method comprises the following steps: (a) binding the nucleic acids to the nucleic acid-binding phase at a pH which is below the pH of the pK of the nucleic acid-binding groups A (binding pH); (b) eluting the nucleic acids at a pH which is above the binding pH (elution pH). In addition, corresponding kits and also nucleic acid-binding phases which can be used for purifying nucleic acids are disclosed. The technology according to the invention permits the purification of nucleic acids and, in particular, elution, with use of low salt concentrations, and so the purified nucleic acids can be directly processed, for example used in a PCR.

The present invention relates to a method for purifying a defined amount of nucleic acids from a nucleic acid-containing sample, which has at least the following steps: (a.) contacting the nucleic acid-containing sample with a defined amount of a nucleic acid binding phase with the following features: (i) the nucleic acid binding phase has nucleic acid binding ligands that have at least one protonatable group; (ii) the nucleic acid binding ligands are bound to a carrier; (iii) the nucleic acid binding phase has a surface with a low charge density, wherein the amount of nucleic acids in the sample exceeds the binding capacity of the amount of nucleic acid binding phase used; (b.) binding of the nucleic acids to the nucleic acid binding phase at a pH (binding pH) that is below the pKs value of at least one of the protonatable groups; (c.) elution of the nucleic acids at a pH that is above the binding pH, wherein a defined amount of nucleic acids is obtained. Furthermore, corresponding kits and nucleic acid binding phases, which can be used for the purification of nucleic acids, are disclosed.

The present invention provides copolymers that facilitate nucleic acid analysis, compositions that comprise such copolymers, and methods for making or using such copolymers.

Sample lysis and coating of reaction surface

The invention concerns a method for the detection of cytosine methylations in DNA and a kit for undertaking an assay according to the method of the invention.

Process for the detection of cytosine methylations

Porous, ferro- or ferrimagnetic, glass particles are described that selectively bind molecules of interest, especially nucleic acid molecules; under appropriate conditions. Methods of preparing the porous, ferro- or ferrimagnetic, glass particles and their use for identifying or separating molecules of interest are also described. Kits comprising the porous, ferro- or ferrimagnetic, glass particles are also provided.

Porous ferro-or ferrimagnetic glass particles for isolating molecules

Porous, ferro- or ferrimagnetic, glass particles are described that selectively bind molecules of interest, especially nucleic acid molecules, under appropriate conditions. Methods of preparing the porous, ferro- or ferrimagnetic, glass particles and their use for identifying or separating molecules of interest are also described. Kits comprising the porous, ferro- or ferrimagnetic, glass particles are also provided.

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Leverkusen, Germany

Roland Fabis