Amgen Inc.

Atara Biotherapeutics, Inc.

Rohini Deshpande

Thomas Charles Boone

Jeonghoon Sun

Yue-Sheng Li

Mark Leo Michaels

Lei-Ting Tony Tam

Huiquan Han

Leslie Phillip Miranda

Arvia Eleanor Morris

Hq Han

Rebecca E McCoy

Sohye Kang

Chetan Goudar

Hedieh Barkhordarian

Yan Wang

Natalia Gomez

The present invention provides a continuous upstream manufacturing process for the production of bispecific antibody products, which comprise at least two binding domains. The process comprises at least the steps of (i) providing in a perfusion bioreactor at least one mammalian cell culture, which is capable of expressing the bispecific antibody product, (ii) growing the mammalian cell culture at a first perfusion rate until a set point viable cell density is reached, and (iii) maintaining perfusion culture at a second perfusion rate, wherein the bispecific antibody product concentration in the bioreactor is kept below a threshold value. The bispecific antibody product is then subject to subsequent downstream processing. Moreover, the invention provides a bispecific antibody product produced by the continuous upstream manufacturing process.

Continuous manufacturing process for bispecific antibody products

The present invention provides stabilized activin IIB receptor polypeptides and proteins capable of binding and inhibiting the activities of activin A, myostatin, or GDF-11. The present invention also provides polynucleotides, vectors and host cells capable of producing the stabilized polypeptides and proteins. Compositions and methods for treating muscle-wasting diseases and metabolic disorders are also provided.

Stabilized receptor polypeptides and uses thereof

Isolated nucleic acid molecules encoding stabilized receptor polypeptides and uses thereof

The present invention relates to the culture of animal cells in serum-free culture medium. The present invention provides particular dipeptides that can improve recombinant protein production and cell viability in such cultures, especially in the absence of peptones.

Dipeptides to enhance yield and viability from cell cultures

Stabilized activin IIB receptor polypeptides and uses thereof

Growing community of inventors

Camarillo, CA, United States of America

Rohini Deshpande