Genentech, Inc.

Roger Pai

Zahra Shahrokh

Heather L Flores

Tanya P Lin

Timothy C Matthews

Avi J Ashkenazi

Robert F Kelley

Iphigenia Koumenis

Woon-Lam Susan Leung

Laura C Simmons

Sarah G Hymowitz

Mark O'Connell

Evan E Shave

Patricia A Rancatore

Iphegenia Koumenis

Susan Leung

The present invention relates generally to Apo2L/TRAIL purification involving crystallization.

Apo-2 ligand/trail formulations

Methods of making Apo-2 ligand and formulations of Apo-2 ligand using divalent metal ions are provided. Such divalent metal ions include zinc and cobalt which improve Apo-2 ligand trimer formation and stability. The crystal structure of Apo-2 ligand is also provided, along with Apo-2 ligand variant polypeptides identified using oligonucleotide-directed mutagenesis.

Methods for making Apo-2 ligand using divalent metal ions

The inventions include Apo2L/TRAIL formulations and methods of using such formulations. Lyophilized and crystal formulations of Apo-2L/TRAIL which are stable and have improved Apo2L/TRAIL trimer formation are provided. Methods of making Apo-2L/TRAIL formulations, as well as devices and kits containing such formulations are also provided.

APO-2 ligand/trail formulations

ApO2 ligand/TRAIL formulations

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Los Altos, CA, United States of America

Roger Pai