Bio-Rad Laboratories, Inc.

Chiron Diagnostics Corporation

Ciba Corning Diagnostics Corp.

Bio-Rad Europe Gmbh

Roger P Walker

Ravi Kaul

Weiming Zheng

Benedicte Jardin

Yabin Lu

Victor D Liu

Jeanne P Harvey

Susan L Hammond

Patricia A McDonald

Victoria A Crebbin

Daming Shan

Urvee Desai

Robert K Likuski

John Flanagan

Daniel Laune

Qian Gao

Howard S Cuckle

Shuxia Zhou

Florian Salipante

Isabelle Batxelli-Molina

Jeannette Fareh-Michiel

Emilie Du Paty

Provided herein are multiplex assays for detecting antibodies indicative of presence and stage of syphilis infection in an individual. Individuals infected with syphilis produce antibodies directed to syphilis components and the lipid cellular debris associated with the infection. The present disclosure represents the first combination of these diverse antibody targets in a single assay.

Combination treponemal and non-treponemal syphilis test

The invention provides a method of diagnosing Non-Alcoholic Steatohepatitis (NASH) and/or the hepatic fibrosis status of a subject, especially a subject afflicted with Non-alcoholic fatty liver disease (NAFLD) or NASH, based on the level of only three or more particular biomarkers. The invention further provides a kit suitable for performing said method and the use of said method and methods of treating patients diagnosed in accordance with the disclosed methods.

Biomarker combinations to simultaneously evaluate non-alcoholic steatohepatitis and hepatic fibrosis status

The present invention relates to the development of novel immunoassays for the detection of neutralizing antibodies and/or high avidity neutralizing antibodies to SARS-CoV-2 spike protein variants or fragments thereof and, optionally, one or more cytokine in patient samples. Novel multiplex and singleplex immunoassays for the detection of neutralizing antibodies and/or high avidity neutralizing antibodies to SARS-CoV-2 spike protein variants or fragments thereof and, optionally, one or more cytokine in patient samples are also provided.

Immunoassay for SARS-CoV-2 neutralizing antibodies and materials therefor

Hemoglobin, its variants, and glycated forms of each are determined individually in a multiplex assay that permits correction of the measured level of HbA1c to account for glycated variants and other factors related to the inclusion of the variants in the sample. New antibodies that are particularly well adapted to the multiplex assay are also provided.

Multiplex immunoassays for hemoglobin, hemoglobin variants, and glycated forms

Methods of diagnosing celiac disease in a subject are provided. In some embodiments, the method comprises contacting a sample of bodily fluid from the subject with an antigen comprising a recombinant or synthetic deamidated gliadin protein, wherein the deamidated gliadin protein comprises a hexamer of peptides each having the sequence of SEQ ID NO:1; and detecting an antibody from the sample that specifically binds to the antigen, thereby diagnosing celiac disease in the subject.

Recombinant deamidated gliadin antigen

Provided herein are multiplex assays for determining whether an individual is or has been infected with Hepatitis B virus, and the stage of infection or resolution. In addition, the multiplex system can discriminate between vaccinated subjects and subjects susceptible to Hepatitis B infection.

Multiplex hepatitis B assay

The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

Growing community of inventors

Benicia, CA, United States of America

Roger P Walker