The Scripps Research Institute

Donald Danforth Plant Science Center

Other

Calgene Corporation

Roger N Beachy

Shunhong Dai

John H Fitchen

Bertrand Verdaguer

Alexandre De Kochko

Claude M Fauquet

Maria Isabel Ordiz Luis

Stephen G Rogers

Robert T Fraley

Claude Fauguet

Malla Padidam

Ron Gafny

Moshe Lapidot

Silvana Petruccelli

Claude Fauquet

Novel chimeric plant promoter sequences are provided, together with plant gene expression cassettes comprising such sequences. In certain preferred embodiments, the chimeric plant promoters comprise the BoxII cis element and/or derivatives thereof. In addition, novel transcription factors are provided, together with nucleic acid sequences encoding such transcription factors and plant gene expression cassettes comprising such nucleic acid sequences. In certain preferred embodiments, the novel transcription factors comprise the acidic domain, or fragments thereof, of the RF2a transcription factor. Methods for using the chimeric plant promoter sequences and novel transcription factors in regulating the expression of at least one gene of interest are provided, together with transgenic plants comprising such chimeric plant promoter sequences and novel transcription factors.

Methods and compositions for regulating gene expression in plant cells

The invention relates to compositions and methods useful for the production of transgenic plants. In particular, the invention relates to cassava vein mosaic virus (CsVMV) promoter sequences and expression cassettes containing CsVMV promoter sequences. The invention describes nucleic acid molecules, vectors and transgenic plants containing promoters derived from CsVMV promoter that are operatively linked to heterologous DNA sequences, and methods for producing transgenic plants containing these promoters.

Transgenic plant comprising a cassava vein mosaic virus promoter and a heterologous nucleic acid sequence

Novel chimeric plant promoter sequences are provided, together with plant gene expression cassettes comprising such sequences. Novel transcription factors are provided, together with nucleic acid sequences encoding such transcription factors and plant gene expression cassettes comprising such nucleic acid sequences. Methods for using the chimeric plant promoter sequences and novel transcription factors in regulating the expression of at least one gene of interest are provided, together with transgenic plants comprising such chimeric plant promoter sequences and novel transcription factors.

A method of activating the rice tungro bacilliform virus (RTBV) promoter in vivo is disclosed. The RTBV promoter is activated by exposure to at least one protein selected from the group consisting of Rf2a and Rf2b.

Rf2a and rf2b transcription factors

The invention relates to compositions useful for the production of transgenic plants. In particular, the invention relates to Cassava vein mosaic virus (CsVMV) promoter sequences and expression cassettes containing CsVMV promoter sequences. The invention describes nucleic acid molecules, vectors and transgenic plants containing promoters derived from CsVMV promoter that are operatively linked to heterologous DNA sequences, and methods for producing transgenic plants containing these promoters.

Cassava vein mosaic virus promoter nucleic acid sequences and expression vectors

The invention describes methods for producing plant resistance to a ssDNA virus, particularly a geminivirus such as mastrevirus, curtovirus or begomovirus. The method comprises introducing a ssDNA-binding protein of thevirus into the plant, and includes a phage coat protein, particularly, a coliphage gene 5 protein. The invention also describes a transgenic plant comprising a gene that expresses the ssDNA-binding protein and vectors for expressing the protein in plants.

Resistance in plants to infection by ssDNA virus using inoviridae virus ssDNA-binding protein, compositions and methods of use

The invention involves recombinant, double-stranded DNA that contains a promoter which functions in plant cells to cause the production of RNA sequences of a plant virus, a DNA sequence that causes the production of an RNA sequence encoding the coat protein of said plant virus, and a 3&prime; non-translated region which functions in plant cells to cause the addition of polyadenylated nucleotides to the 3&prime; end of said RNA sequence; which double-stranded DNA can be used in a method for genetically transforming plants to produce genetically transformed plant cells and plants that are resistant to virus infection.

Protection of plants against viral infection

The invention describes compositions and methods of use in which an infectious modified Tobacco Mosaic Virus (TMV) virion comprising a coat protein (CP) or a movement protein (MP) gene is replaced with a nuclear inclusion protease (NIa) expression cassette for the expression of a heterologous peptide in a tobacco mosaic virus (TMV) host plant.

Method for using tobacco mosaic virus to overproduce peptides and proteins

Method for using tobacco mosaic virus to overproduce peptides and

The present invention relates to the methods and nucleic acid compositions for the production of transgenic plants resistant to virus infection. In particular, it relates to transgenic plants comprising nucleotide sequences encoding dysfunctional viral movement protein (dMP) genes.

Growing community of inventors

St. Louis, MO, United States of America

Roger N Beachy