Roche Molecular Systems, Inc.

Roche Sequencing Solutions, Inc.

Genia Technologies, Inc.

Roger Ja Chen

David J Fullagar

Hui Tian

Randall W Davis

William D Nielsen

Ashraf M Wahba

Michael Dorwart

Kevin Aliado

Kevin Deierling

Jing Luo

Kyle Umeda

Jennifer Hovis

Daniel Korenblum

Arkadiusz Bibillo

A nanopore sequencing device is disclosed. The nanopore sequencing device includes a working electrode. It further includes a dielectric layer, wherein a portion of the dielectric layer is disposed horizontally adjacent to the working electrode and a portion of the dielectric layer is disposed above and covering a portion of the working electrode, and wherein the dielectric layer forms a well having an opening above an uncovered portion of the working electrode. A base surface area of the working electrode is greater than a base surface area of the opening above the uncovered portion of the working electrode.

Adjustable bilayer capacitance structure for biomedical devices

A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.

Non-destructive bilayer monitoring using measurement of bilayer response to electrical stimulus

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Saratoga, CA, United States of America

Roger Ja Chen