Genmark Diagnostics, Inc.

Stratagene California

Roche Molecular Systems, Inc.

Other

Roger Harry Taylor

Tyler David Jensen

John B Gorman

Taylor Reid

Larry R Brown

Kenneth J Zajac

Alan Ridgeway Orr

Scott O'Brien

Kyoung Ho Kim

Disclosed are devices, systems and methods for assessing the integrity of electrical connections between elements of interfacing electronic devices. In some aspects, a system includes an analysis device having electronics that interface with an assay cartridge inserted into the analysis device, wherein the analysis device is configured to conduct a preflight test in which impedance values for each circuit between the assay cartridge and analysis device are rearranged and assessed to determine the electrical connection integrity of the assay cartridge to the analysis device prior to implementing the assay.

Systems and methods for assessing electrical connectivity between elements of assay devices

Instrument

A system and method for fluorescence excitation and detection having distinct optical paths is disclosed. A system for detecting fluorescence comprises a light source that emits an excitation light into an illumination tube; a plurality of collection optics located around an aperture in the illumination tube for collecting fluorescence; and a detector for determining the amount of fluorescence. A method for detecting fluorescence comprises emitting an excitation light from a light source into an illumination tube; directing the excitation light to an excitation filter; illuminating a sample with the excitation light to generate an emission light; and detecting the optical characteristics of the emission light using a plurality of collection optics located around the illumination tube.

System and method for a fluorescence excitation and detection having distinct optical paths

The invention relates to an apparatus and methods for preventing condensation on the interior surfaces of sample tubes which are being exposed to temperature cycles, such as during a PCR amplification reaction. In particular, the invention relates to apparatus comprising a sample block comprising a plurality of sample wells for receiving sample tubes and heating elements disposed in the sample wells for heating at least a portion of the sides of sample tubes (e.g., at least the portion which forms the head space after a tube is filled with a PCR reaction mixture). In a preferred aspect, the sample block is part of a thermocycling device for performing PCR and the side-wall heater is used to enhance uniformity and speed of amplification reactions. For example, by decreasing or eliminating condensation, signal strength jumps in a real-time PCR assay can be minimized as can reaction non-homogeneity.

Side-wall heater for thermocycler device

The invention relates to baseline subtraction algorithms developed to reduce tube-to-tube and cycle-to-cycle variabilities during real time PCR amplification. Particularly, the invention relates to algorithms for determining the threshold cycle for the first reliable detection of the amplified nucleic acid product. The invention also relates to computer programs comprising the algorithm and methods and/systems implementing the algorithm.

Adaptive baseline algorithm for quantitative PCR

A baseball batting practice device is disclosed as a bat having a recess formed at the large end to nestingly receive a spinning reel. The reel is oriented to pay-out a fishing line at a right angle to the bat axis, the fishing line is secured to a baseball and serves as a tether to retrieve the ball using the reel. With the tether payed out at a right angle to the bat axis, minimum resistance to the flight of the ball is observed and the distance traveled by the ball replicates that of an untethered ball.

Growing community of inventors

San Diego, CA, United States of America

Roger Harry Taylor