Synthecon, Inc.

Roger Akers

Stephen S Navran, Jr

William J Anderson

Adrian F Dinges, Jr

The culture chamber of the present invention has a fluid-filled culture compartment in which cells, tissues and other biologicals are cultured. The culture compartment is transversed by one or more molecular weight cut-off membranes attached to a membrane carrier assembly. Incoming nutrients are transported through the membrane into the culture compartment and metabolic waste products are transported away from the fluid-filled culture compartment through the membrane and out the chamber outlet. Both reusable and disposable culture chambers are described for culturing cells, cell aggregates, particles, tissues and organoids.

Culture chamber for biologicals

The bioreactor system of the present invention has two fluid-filled culture compartments in which cells, tissues and other biologicals are cultured. The two culture compartments are in fluid communication with each other and each culture compartment is transversed by a filter that prevents the exit of the cells from the culture compartment. Both reusable and disposable culture chambers are described for use as the first and/or second culture compartment.

Interlinked culture chamber for biologicals

A process has been developed for the production of human recombinant polypeptides using transformed mammalian cells cultured in a horizontally rotating culture vessel modulated to create low shear, high mass transfer conditions. The resulting recombinant polypeptide has bioactivity and is produced in increase amounts.

Methods for efficient production of mammalian recombinant proteins

A cell culture bag for culturing cells, cellular aggregates, particles, tissues and organoids is disclosed. The cell culture bag is a flexible and disposable culture bag that fits within a bag support chamber of a bioreactor. The cell culture bag has an inlet means and an outlet means for the introduction and removal of media. Certain embodiments of the culture bag have an inlet and outlet fluid coupling swivel joint and/or perforated perfusion tubes extending from the inlet means into the interior of the bag to assist in mass transfer and mixing the media to maintain an even temperature throughout the culture bag.

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Houston, TX, United States of America

Roger Akers