E.i. Dupont De Nemours and Company

Dupont Industrial Biosciences Usa, LLC

Rodrigo Ramirez

Robert DiCosimo

Jayarama K Shetty

Susan Marie Hennessey

Zheyong Yu

Kevin D Nagy

Ling Hua

Zhongmei Tang

Erwin F Columbus-Hago

Erwin Columbus Hago

A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example.

Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

A method is disclosed for hydrolyzing an alpha-1,5 glucosyl-fructose linkage in a saccharide (disaccharide or oligosaccharide) such as leucrose. This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase or glucoamylase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose linkage of the saccharide. This method is useful for reducing the amount of leucrose in a filtrate isolated from a glucan synthesis reaction, for example.

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Campinas, Brazil

Rodrigo Ramirez