Palo Alto Research Center Inc.

Evotec Biosystems Ag

Rodney Turner

Francisco Eduardo Torres

Richard H Bruce

Karsten Henco

Rolf Guenther

Juergen Rolf Mueller

Peter Axhausen

A method for screening of compounds for binding differentiation at various drug target binding sites is used with a device measuring the enthalpy of reaction for the binding. The method includes merging test ligand with target compound and merging test ligand with target compound in the presence of at least one blocking agent. A first heat of reaction is detected for the merged test ligand and target compound solution and a second heat of reaction is detected for the merged test ligand and target compound solution in the presence of a blocking agent. The two heats of reaction are compared to determine whether a reaction has occurred.

Screening for ligand binding at specific target sites

The invention relates to a method for optically detecting at least one entity which is arranged on a substrate. The at least one entity is scanned with a measuring volume using at least one radiation source and a confocal optic. During a scanning process an auxiliary focus is generated by means of at least one second radiation source and a second optic. Radiation generated by the first radiation source is collimated by a first optic and radiation generated by the second radiation source is collimated by a second optic. A retroreflection from the auxiliary focus is detected by at least one detector and is used to measuring the position of an interface and, thus, for indirectly positioning the measuring volume. The position of the auxiliary focus relative to the measuring volume is adjustable in a defined manner.

Scanning microscopic method having high axial resolution

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Rodney Turner