Wisconsin Alumni Research Foundation

Other

Rodney A Welch

Wyndham W Lathem

Thomas E Grys

Patrick J Dillon

Gil H Choi

Gary A Splitter

Laura Walters

Sarah S Hurley

Paula L Roesch

Disclosed is a pO157 plasmid-specified polypeptide found inEDL933 and otherthat binds to and cleaves C1-esterase inhibitor, and antibodies specific for the polypeptide. Also disclosed are methods employing the polypeptide for diagnosing enterohemorrhagicinfection, identifying potential inhibitors of its activity, and reducing viscosity of material containing glycosylated polypeptides.

Method of reducing the viscosity of mucus

Disclosed are methods of detecting uropathogenicgenes that are differentially expressed in response to D-serine. Also disclosed are methods of characterizing bacterial isolates from clinical samples based on the ability to metabolize D-serine.

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Method of reducing complement - mediated disruption of cells

Disclosed is a pO157 plasmid-specified polypeptide found inEDL933and other enterohemorrhagicthat binds to and cleaves C1-esterase inhibitor. Also disclosed are methods employing the polypeptide for diagnosing and treating colitis or hemolytic uremic syndrome, and methods of detecting potential therapeutics.

E. coli O157:H7 C1 esterase inhibitor-binding protein and methods of use

The present invention relates to novel genes located in two chromosomal regions within uropathogenic that are associated with virulence. These chromosomal regions are known as pathogenicity islands (PAIs). In particular, the present application discloses 142 sequenced fragments (contigs) of DNA from two pools of cosmids covering pathogenicity islands PAI IV and PAI V located on the chromosome of the uropathogenic J96. Further disclosed are 351 predicted protein-coding open reading frames within the sequenced fragments.

Nucleotide sequence of Escherichia coli pathogenicity islands

The present invention relates to novel genes located in two chromosomal regions within uropathogenic,that are associated with virulence. These chromosomal regions are known as pathogenicity islands (PAIs). In particular, the present application discloses 142 sequenced fragments (contigs) of DNA from two pools of cosmids covering pathogenicity islands PAI IV and PAI V located on the chromosome of the uropathogenic,J96. Further disclosed are 351 predicted protein-coding open reading frames within the sequenced fragments.

A test to diagnose the presence of Johne's disease is disclosed. In one aspect it involves using a DNA segment that will hybridize to M. paratuberculosis but not to other bacteria typically found in bovine feces. Another aspect of the invention is providing a process for breaking the cell wall of mycobacteria using dense beads in the presence of phenol.

Growing community of inventors

Madison, WI, United States of America

Rodney A Welch