Promega Corporation

Roderick G Flemming

Daniel J Simpson

Tonny Johnson

Mary P Hall

Poncho Meisenheimer

John William Shultz

Wenhui Zhou

Thomas Machleidt

Matthew B Robers

Joel R Walker

Nidhi Nath

Rex M Bitner

Robin Hurst

Rebecca Godat

Susan C Koller

Sanchayita Kar

Compounds that may inhibit-derived luciferases are disclosed as well as compositions and kits comprising the compounds, and methods of using the compounds.

Functionalized NANOLUC inhibitors

The present invention provides compositions and methods for the separation of metals or molecules such as polypeptides, nucleic acids, or endotoxins using a metal-modified solid support. The metals or molecules are isolated from a starting material using the modified solid supports of the invention. Also provided by the invention are kits that can be used in connection with the inventive methods.

Methods for separating molecules

The present invention provides compositions for the separation of metals or biomolecules such as polypeptides, nucleic acids, or endotoxins using modified solid supports.

Compositions for separating molecules

A method is provided for using a silanized silica matrix to isolate a target nucleic acids, such as plasmid DNA, fragments of DNA, chromosomal DNA, or RNA from contaminants, including proteins, lipids, cellular debris, or non-target nucleic acids. The silanized silica matrix comprises a silica based solid phase and a plurality of silane ligands covalently attached to the surface of the solid phase. Non-target material adsorbs to the silanized silica matrix in the presence of a sufficient concentration of chaotropic salt, while target nucleic acids adsorb to the matrix under other solution conditions. The method of using the silanized silica matrix of the present invention can be used to clear solutions of disrupted biological material, and to isolate nucleic acids therefrom or from other solutions containing nucleic acids and at least one contaminant.

Lysate clearance and nucleic acid isolation using silanized silica matrices

Growing community of inventors

McFarland, WI, United States of America

Roderick G Flemming