.Kemin Foods, L.c.

Kemin Consumer Care, L.c.

Rod Ausich

Robert Stomp

Fayad Z Sheabar

Clarence A Ryan

Zoraida DeFreitas

Jerry Newman

Hal G Fallert

Anthony W Newman

Amanda Ueltschy

Jennifer Barker

George Mather

Brent Davieson

Brent Davidson

Holly Johnson

Concentrated solutions of carotenoids in essential oils and cosmetic oils for use in foods, beverages, and personal care products. The concentrated solutions comprise between about 0.8 weight % and about 8 weight % carotenoid in the oil, or between about 15 times and about 100 times the maximum concentration reported in the prior art. The concentrated solutions are particularly suited for adding carotenoids to foods, beverages, and personal care products. An antioxidant may be added to help in preventing oxidation of the carotenoid.

Solutions of carotenoids for use in foods and personal care products

The present invention provides a method for the extraction of a proteinase inhibitor from plant tissue. The extraction of the proteinase inhibitor begins with the addition of an alcohol-free, aqueous solution of an organic acid and a salt to plant tissue. The extraction solution and plant tissue are comminuted to reduce the average particle size of the plant tissue to improve extraction efficiencies. A weight ratio of between about 1:1 and about 1:10 extraction solution to plant tissue is used. In extracting proteinase inhibitor II from potato tubers, the extraction solution utilizes formic acid and sodium chloride, and the average particle size is reduced to between about 100 and 1500 microns. The process has been demonstrated to be cost-effective and provide high yields of the target proteinase inhibitor on commercial scales.

Method of enhancing the extraction of proteinase inhibitors

A method for removing protein impurities from extracts of protease inhibitor-containing plant material. Plant materials containing protease inhibitors, such a potato tubers that contain protease inhibitor II, are extracted using an alcohol-free solvent. The proteins present in the extract include impurities other than the protease inhibitor, specifically Kunitz, Bowman-Birk and carboxypeptidase inhibitors. The extract is subjected to heat treatment to denature and precipitate the unstable protein impurities followed by centrifugation to remove the precipitate. Ultrafiltration in the presence of a buffer removes the Bowman-Birk and carboxypeptidase inhibitors. The resulting purified protease inhibitor has applicability in the control of obesity and diabetes.

Method for the elimination of Kunitz and Bowman-Birk trypsin inhibitors and carboxypeptidase inhibitor from potato proteins

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Des Moines, IA, United States of America

Rod Ausich