Dow Agrosciences LLC

Mycogen Corporation

Agrigenetics, Inc.

Rod Aaron Herman

George E Schwab

Barry W Schafer

Kenneth Edwin Narva

H Ernest Schnepf

Donald J Merlo

Guy A Cardineau

Mark E Knuth

Tracy Ellis Michaels

Michael R Pollard

Lining Guo

Ann Owens Merlo

James K Petell

Stacey Finstad Lee

Lisa M Stamp

Jean L Roberts

Kitisri Sukhapinda

Joanna Dojillo

Deborah Ann Schwedler

Xioaping Xu

Andrew William Carr

Yong Gao

Paula Diehl

The invention provides a method for devitalizing plant seed, the method comprising the steps of hydrating a viable whole plant seed and freezing the hydrated whole plant seed. The invention further provides a collection of devitalized whole plant seed wherein the integrity of genomic DNA and protein within the devitalized plant seed is preserved.

Method for seed devitalization

This invention provides truncated Cry 35 proteins that surprisingly and unexpectedly have increased pesticidal activity as compared to full-length Cry 35 proteins. The subject invention also includes polynucleotides that encode these truncated proteins, transgenic plants comprising a truncated gene of the subject invention, and transgenic plants that produce these truncated proteins. This invention further provides methods of controlling plant pests, including rootworms, with these truncated proteins. The truncated Cry35 proteins of the subject invention are preferably used in combination with Cry34 proteins, which are known in the art. Various surprising advantages of the subject invention will be apparent in light of this disclosure.

Truncated Cry35 proteins

This invention provides truncated Cry35 proteins that surprisingly and unexpectedly have increased pesticidal activity as compared to full-length Cry35 proteins. The subject invention also includes polynucleotides that encode these truncated proteins, transgenic plants comprising a truncated gene of the subject invention, and transgenic plants that produce these truncated proteins. This invention further provides methods of controlling plant pests, including rootworms, with these truncated proteins. The truncated Cry35 proteins of the subject invention are preferably used in combination with Cry34 proteins, which are known in the art. Various surprising advantages of the subject invention will be apparent in light of this disclosure.

The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences that encode these proteins. In preferred embodiments, these pesticidal protein have molecular weights of approximately 40&#x2013;50 kDa and of approximately 10&#x2013;15 kDa.

Pesticidal proteins

Novel polynucleotide sequences that encode insect toxins TcdA and TcbA have base compositions that differ substantially from the native genes, making them more similar to plant genes. The new sequences are suitable for use for high expression in both monocots and dicots. Transgenic plants with a genome comprising the nucleic acid of SEQ ID NO:4 are insect resistant.

Transgenic plants expressing photorhabdus toxin

The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences that encode these proteins. In preferred embodiments, these pesticidal proteins have molecular weights of approximately 40-50 kDa and of approximately 10-15 kDa.

Novel polynucleotide sequences that encode insect toxins TcdA and TcbA have base compositions that differ substantially from the native genes, making them more similar to plant genes. The new sequences are suitable for use for high expression in both monocots and dicots. Transgenic plants with a genome comprising a nucleic acid of SEQ ID NO: 3 or SEQ ID NO:4 are insect resistant.

Growing community of inventors

New Ross, IN, United States of America

Rod Aaron Herman