Roche Molecular Systems, Inc.

Rochak Mehta

Jenny A Johnson

Jingtao Sun

Kyle C Cady

Patrick Lin

Troy Rabang

Brett Hanson

Paulino Abdon

Andy Yuen

Ryan Chan

The present invention relates to compositions and methods for the use of polymerase chain reaction (PCR) as a reporter assay for rapid and simultaneous bacterial identification and phenotype testing for antimicrobial susceptibility (AST). The current invention uses a strategy that has shown the ability for multiplexing and for handling polymicrobial samples for antimicrobial susceptibility testing.

Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi

Methods for the rapid detection of the presence or absence of BK virus in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting BK virus and kits are provided that are designed for the detection of BK virus.

Compositions and methods for detection of BK virus

Methods for the rapid detection of the presence or absence of(MTB) resistant to rifampicin (MTB-RIF) and/or MTB resistant to isoniazid (MTB-INH) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the genes for rpoB, inhA, and katG, along with kits are provided that are designed for the detection of MTB-RIF and/or MTB-INH.

Compositions and methods for detection of drug resistant

Improved methods for the detection and quantitation of a target nucleic acid in a sample using a non-extending helper oligonucleotide are described. The methods include contacting nucleic acids in a sample with amplification reagents including one or more primers, one or more non-extending helper oligonucleotides, and one or more probes. The non-extending helper oligonucleotide facilitates and increases the target nucleic acid accessibility of one or more of the primers, result in greater accumulation of amplicon production, thereby increasing the efficiency and sensitivity of the amplification assay, including amplification assays for Hepatitis C Virus (HCV), for example, HCV Genotype 5. Kits, articles of manufacture, and reaction mixtures are also provided.

Helper oligonucleotide for improved efficiency of amplification and detection/quantitation of nucleic acids

Methods for the rapid detection of the presence or absence of Hepatitis C Virus (HCV) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting HCV and kits are provided that are designed for the detection of HCV.

Compositions and methods for detection of hepatitis C virus genotype 3

SNP specific hydrolysis probe including a hairpin structure toward the 3' end, along with kits are provided that are designed for the detection of a SNP in a target nucleic acid.

Detecting single nucleotide polymorphism using hydrolysis probes with 3' hairpin structure

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Methods for the rapid detection of the presence or absence of a SNP in a target nucleic acid in a sample are described. The methods can include performing an amplifying step, a hybridizing step utilizing a double stranded probe with two overlapping SNP specific hydrolysis probe sequences where one of the probe sequences can include a hairpin structure toward the 3' end, and a detecting step. Furthermore, the double stranded SNP specific hydrolysis probes along with kits are provided that are designed for the detection of a SNP in a target nucleic acid.

Detecting single nucleotide polymorphism using overlapping hydrolysis probes

Methods for the rapid detection of the presence or absence of a SNP in a target nucleic acid in a sample are described. The methods can include performing an amplifying step, a hybridizing step utilizing a SNP specific hydrolysis probe including a hairpin structure toward the 3' end, and a detecting step. Furthermore, SNP specific hydrolysis probe including a hairpin structure toward the 3&#x2032; end, along with kits are provided that are designed for the detection of a SNP in a target nucleic acid.

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Union City, CA, United States of America

Rochak Mehta