Commonwealth Scientific and Industrial Research Organisation

Robyn Joyce Russell

John Graham Oakeshott

Peter Malcolm Campbell

Richard David Newcomb

Susan Jane Dorrian

Thomas Mark Boyce

Tara Sutherland

Irene Horne

Rama Heidari

Alan Devonshire

Geoffrey Charles De Quetteville Robin

Charles Claudianos

Rebecca Harcourt

Jeremy Colin Brownlie

Kerrie-Ann Smyth

Steven Kotsonis

Kaiyan Liu

Chris M Coppin

Gunjan Pandey

Kerrie-Anne Smyth

Nigel French

Colin Scott

Geoffrey Charles Robin

Anthony Gerard Parker

The present invention relates to enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention relates to variants of the OpdA enzyme fromthat display improved activity when compared to the naturally occurring OpdA. The invention is also towards polypeptides that have organophosphate hydrolysing activity for the organophosphates chlorpyrifos methyl, diazinon and parathion ethyl.

Enzymes for degrading organophosphates

The present invention provides enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention provides a phosphotriesterase enzyme identified from anstrain isolated from soil that hydrolyses OP pesticides, and the gene encoding that enzyme. The invention also provides mutants of the identified phosphotriesterase enzyme which have altered substrate specificity. The use of these enzymes in bioremediation strategies is also provided.

Phosphotriesterase from agrobacterium radiobacter P230

Phosphotriesterase from agrobacterium radiobacter p230

The invention relates to bacteria, bacterial extracts, supernatants obtained from the culturing of said bacteria, polypeptides and compositions for degrading benzimidazole carbamate fungicides, carbanilate fungicides, sulfonamide herbicides, thioamide herbicides and/or synthetic pyrethroid insecticides. In particular, the invention relates to the identification ofsp. which degrades benzimidazole carbamate fungicides, carbanilate fungicides, sulfonamide herbicides, thioamide herbicides and/or synthetic pyrethroid insecticides.

Methods for degrading toxic compounds

The present invention relates to methods for degrading hydrophobic ester pesticides and toxins. In particular, the present invention relates to the use of insect esterases, and mutants thereof, in the bioremediation of hydrophobic ester pesticides and toxins residues, such as pyrethroid residues, contaminating the environment and horticultural commodities.

Degradation of hydrophobic ester pesticides and toxins

The present invention relates to methods and enzymes for degrading hydrophobic ester pesticides and toxins. In particular, the present invention relates to the use of insect esterases, and mutants thereof, in the bioremediation of hydrophobic ester pesticides and toxins residues, such as pyrethroid residues, contaminating the environment and horticultural commodities.

The present invention provides an isolated DNA molecule encoding Malathion Carboxylesterase capable of hydrolysing at least one organophosphate selected from the group consisting of carboxylester organophosphates and dimethyloxon organophosphates. The DNA molecule comprises a nucleotide sequence having at least 60%, preferably at least 80% and more preferably at least 95% homology with Lc?E 7, in which the protein encoded by the DNA molecule differs from E 3 at least in the substitution of Trip at position 251 with an amino acid selected from the group consisting of Leu, Ser, Ala, Ile, Val, Thr, Cys, Met and Gly. The preferred substituents are Leu and Ser.

Malathion carboxylesterase

The present invention provides an isolated DNA molecule encoding Malathion Carboxylesterase capable of hydrolysing at least one organophosphate selected from the group consisting of carboxylester organophosphates and dimethyloxon organophosphates. The DNA molecule comprises a nucleotide sequence having at least 60%, preferably at least 80% and more preferably at least 95% homology with Lc&agr;E,, in which the protein encoded by the DNA molecule differs from E,at least in the substitution of Trip at position,with an amino acid selected from the group consisting of Leu, Ser, Ala, Ile, Val, Thr, Cys, Met and Gly. The preferred substituents are Leu and Ser.

Enzyme based bioremediation

Growing community of inventors

Wanniassa, Australia

Robyn Joyce Russell