Dana-farber-cancer Institute Inc.

Children's Medical Center Corporation

The Broad Institute, Inc.

Instituto Carlos Slim De La Salud, A.c.

Robin M Meyers

William C Hahn

Frederick W Alt

Aviad Tsherniak

David Root

Andrew J Aguirre

April Cook

Jiazhi Hu

Glenn Cowley

Barbara Weir

Francisca Vazquez

Richard L Frock

The present invention relates to compositions and methods for targeting cancer-specific DNA sequences, such as copy number amplifications and other types of cancer-specific sequence variations, such as cancer-specific polymorphisms, insertions, or deletions. The present invention provides hereto sequence-specific DNA targeting agents targeting a sequence within the amplified DNA region or a sequence otherwise specific for a cancer cell compared to a non-cancer cell. The invention further relates to methods for treating cancer, comprising administering such sequence-specific DNA targeting agents. The invention further relates to methods for preparing sequence-specific DNA targeting agent, as well as screening methods using the DNA targeting agents.

Compositions and methods for targeting cancer-specific sequence variations

Described herein are methods and systems relating to high throughput, genome-wide translocation sequencing (HTGTS) and/or detection of double-stranded DNA break (DSB) locations. The methods described herein can comprise generating DSBs in a nucleic acid sequence and performing nested PCR with the primers described herein. Described herein is an enhanced HTGTS approach. The assays and methods described herein permit the measurement of various DNA double-strand break (DSB) activities either intrinsic to the biological system or from outside agents.

Methods relating to the detection of recurrent and non-specific double strand breaks in the genome

Growing community of inventors

Cambridge, MA, United States of America

Robin M Meyers