Pacific Biosciences of California, Inc.

Pioneer Hi-bred International, Inc.

Maxygen, Inc.

Codexis Mayflower Holdings, LLC

Pioneer International, Inc.

Augmenta Biosciences, Inc.

Robin A Emig

Lei Jia

Jeremy S Minshull

Claes Gustafsson

Sridhar Govindarajan

Fred C Christians

Phillip A Patten

Kevin E McBride

Keith P Bjornson

Takashi Yamamoto

Richard John Fox

Ericka Bermudez

S Christopher Davis

Arek Bibillo

Stephen Wilson Turner

Molly Min He

Erik Miller

Satwik Kamtekar

Linda A Castle

Rebecca Lynne Gorton

Insil Park

Daniel Siehl

Anthony Cox

Teddy Chen

Ajoy K Roy

Jeremiah Hanes

Kevin Travers

Walter H Lee

Harold Ray Lee

Jonas Korlach

Lubomir Sebo

Benjamin A Flusberg

Willem P C Stemmer

Brian S Carr

Gene W Shen

Lorraine Joan Giver

Mark D Welch

Steven Henry Bass

John S Eid

Paul S Peluso

Matthew B Tobin

Andreas Crameri

Jon M Sorenson

Adrian Nielsen Fehr

Joseph A Affholter

Geoffrey Alan Otto

Xiangxu Kong

Sonya Clark

Sun Ai Raillard

Stanley Goldman

Robert Sebra

Pascal Longchamp

Guy Cavet

Kevin Mcbride

Jon Edward Ness

John Lyle

Mathieu Foquet

Andrei Fedorov

Patrick Marks

Donald M Gray

Jeremy Gray

Stephane J Jenne

Stephan Jenne

Lei Sun

Colleen Cutcliffe

Kenneth Mark Maxham

Stephen P Dudek

Hans Callebaut

Steven Lin

Simon Christopher Davis

Bidhan P Chaudhuri

Mark Chaisson

Christopher S Davis

Christopher J Emig

Marco Antonio Mena

Steven C Henry

Payam Shahi

John Beaber

Ravi Dalal

Claus Gustafsson

Daniel L Siehl

John M Lyle

Rosanna Man Wah Chau

Kim-Xuan Nguyen

YuShuan Lai

Jeremy Minshull

Phil Patten

Sridar Govindarajan

Ajoy Apt120 Roy

Rebecca Lynne Apt819 Gorton

The disclosure relates generally to the field of immune binding proteins and method for obtaining immune binding proteins from genomic or other sources. The disclosure also relates to anti-SARS-CoV-2 antibodies that have been obtained from subjects who became immune to this coronavirus, and to methods of using these anti-SARS-CoV-2 antibodies. Methods for neutralizing SARS-CoV-2 are disclosed, as well treatments for SARS-COV-2 using the anti-SARS-CoV-2 antibodies.

Antibodies for SARS-CoV-2 and uses thereof

Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

Recombinant polymerases for improved single molecule sequencing

The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention additionally provides digital systems and software for performing these methods.

Methods, systems, and software for identifying functional bio-molecules

Methods, Compositions, and Systems are provided for nucleic acid sequencing where the sequential incorporation of nucleotides uses two distinct chemical steps. A plurality of nucleotide analogs, each having a labeled leaving group at its 3' hydroxyl can be sequentially added to a growing strand in the presence of a selective cleaving activity that cleaves the 3&#x2032; hydroxyl leaving group preferentially after it has been incorporated. The selective cleaving agent can comprise an exonuclease activity, and the exonuclease activity can be a polymerase-associated exonuclease activity. Nucleotide analogs having labels on both a cleavable polyphosphate portion and on a 3&#x2032; hydroxyl leaving group can provide signals characteristic of nucleotide analog incorporation. Systems having illumination optics, collection optics, and substrates observe signals from the labels as they are being incorporated into a growing nucleic acid strand, allowing for the sequencing of template nucleic acids.

Single molecule sequencing with two distinct chemistry steps

Provided are methods for sequencing a nucleic acid with a sequencing enzyme, e.g., a polymerase or exonuclease. The sequencing enzyme can optionally be exchanged with a second sequencing enzyme, which continues the sequencing of the nucleic acid. In certain embodiments, a template is fixed to a surface through a template localizing moiety. The template localizing moiety can optionally anneal with the nucleic acid and/or associate with the sequencing enzyme. Also provided are compositions comprising a nucleic acid and a first sequencing enzyme, which can sequence the nucleic acid and optionally exchange with a second sequencing enzyme present in the composition. Compositions in which a template localizing moiety is immobilized on a surface are provided. Also provided are methods for using data from analytical reactions wherein two different enzymes are employed, e.g., at a same or different reaction regions.

Nucleic acid sequence analysis

The present invention provides methods and compositions for performing illuminated reactions, particularly sequencing reactions, while mitigating and/or preventing photodamage to reactants that can result from prolonged illumination. In particular, the invention provides methods and compositions for incorporating photoprotective agents into conjugates comprising reporter molecules and nucleoside polyphosphates.

CIS reactive oxygen quenchers integrated into linkers

The invention relates to devices and methods for nanopore sequencing. The invention includes arrays of nanopores having incorporated electronic circuits, for example, in CMOS. In some cases, the arrays of nanopores comprise resistive openings for isolating the electronic signals for improved sequencing. Methods for controlling translocation of through the nanopore are disclosed.

Nanopore sequencing devices and methods

Methods for non-random loading of single analyte molecules into array structures are provided. The methods allow for distribution of a population of target molecules into a plurality of size confined regions such as wells. Sizing moieties are linked to individual target molecules. The sizing moieties are of sufficient size, relative to the size-confined reaction or observation regions, such that only a selected number of sizing moieties will fit into the size confined regions. The confined regions and the sizing moieties or target molecules comprise a selected charge that allow for controlling the loading of the sizing moities.

Single molecule loading methods and compositions

Methods, systems, and software for identifying functional biomolecules

Growing community of inventors

Redwood City, CA, United States of America

Robin A Emig