Stichting Dienst Landbouwkundig Onderzoek

Id-lelystad, Instituut Voor Dierhouderij En Diergezondheid B.v.

Stichting Centraal Diergeneeskundig Instituut

Other

Bayer Aktiengesellschaft

Boehringer Ingelheim Vetmedica Gmbh

Stichting Wageningen Research

Stichting Instituut Voor Dierhouderij En Diergezondheid

Instituut Voor Dierhouderij En Diergezondheid

Stichting Institut Voor Dierhouderij En Diergezonheld

Robertus Jacobus Maria Moormann

Petrus Antonius Van Rijn

Catharinus Terpstra

Gert Wensvoort

Johanna Jacoba Maria Meulenberg

Joannes Maria Anthonis Pol

Jeroen Alexander Kortekaas

Dirk Franciscus Marinus Van De Wiel

Myra Noorely Widjojoatmodjo

Dietmar Kretzdorn

Abraham Johannes De Smit

Erik Kees Hamann

Robert Hans Meloen

Paulus-Jozef Wichgers Schreur

Nadia Dimitrova Oreshkova

The invention relates to a bunyavirus, in which separated (NSm)Gn and Gc coding regions are functionally present on two different genome segments, preferably a bunyavirus that comprises a total of at least 4 genome segments. The invention further relates to methods for producing said bunyavirus, and to a composition comprising said bunyavirus and a suitable excipient.

Bunyaviruses with segmented glycoprotein precursor genes and methods for generating these viruses

The invention relates to methods of producing infectious bunyavirus replicon particles. These bunyavirus replicon particles are safe and can be used outside a biosafety containment. The invention further relates to recombinant bunyavirus replicon particles and uses of these recombinant bunyavirus replicon particles.

Methods to produce bunyavirus replicon particles

The invention relates to vaccines used in the eradication or control of pestivirus infections, particularly those used in pigs or ruminants. The invention provides nucleic acid, pestivirus-like particles and a pestivirus vaccine, comprising the nucleic acid or particles, which is capable of eliciting a proper immune response without having the ability to spread throughout the vaccinated animal, thereby avoiding the negative consequences of viral spread. Preferably, the immune response allows for serological discrimination between vaccinated animals and wild-type pestivirus infected animals.

Non-spreading pestivirus

Composition of matter comprising the causative agent of Mystery Swine Disease, Lelystad Agent, in a live, attenuated, dead, or recombinant form, or a part or component of it. Vaccine compositions and diagnostic kits based thereon. Recombinant nucleic acid comprising a Lelystad Agent-specific nucleotide sequence. Peptides comprising a Lelystad Agent-specific amino acid sequence. Lelystad Agent-specific antibodies.

Causative agent of the Mystery Swine Disease, vaccine compositions and diagnostic kits

The invention relates to vaccines used in the eradication or control of pestivirus infections, particularly used in pigs or ruminants. The invention provides nucleic acid, pestivirus-like particles and a pestivirus vaccine, comprising the nucleic acid or particles, which is capable of eliciting a proper immune response without having the ability to spread throughout the vaccinated animal, thereby avoiding the negative consequences of viral spread. Preferably, the immunological response allows for serological discrimination between vaccinated animals and wild-type pestivirus infected animals.

The invention relates to a method of increasing protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1&#xd7;10to 5&#xd7;10cells/ml with an m.o.i of &#x3c;0.01. The invention also provides a method to produce recombinant pestivirus E2 or Eprotein or fragments thereof in insect cell culture characterized by a final concentration of the protein fragments in the growth medium at harvest of at least 100 &#x3bc;g/ml. The invention also provides a method of producing recombinant FSH, &#x3b1;-units and/or &#x3b2;-units, and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 &#x3bc;/ml.

E2 subunit vaccine comprising recombinant pestivirus E2 protein

Causative agent of the mystery swine disease, vaccine compositions and diagnostic kits

The invention relates to a method to increase protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect-cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1&times;10 to 5&times;10 cells/ml with an m.o.i of <0.01. The invention furthermore provides a method to produce recombinant pestivirus E2 or E protein or fragments thereof in insect cell culture characterized by a final concentration of said protein (fragments) in the growth medium at harvest of at least 100 &mgr;g/ml. The invention furthermore provides a method to produce recombinant follicle stimulating hormone, &agr;-units an/or &bgr;-units and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 &mgr;l.

Protein expression in baculovirus vector expression systems

Mystery swine disease diagnostic kits

The invention provides methods for the production of recombinant bovine Follicle Stimulating Hormone (bFSH) as well as vectors and cells for use in those methods. In particular the invention provides baculovirus based vectors which are capable of expression of bFSH in insect cells. bFSH is a heterodimeric protein belonging to a family of glycoprotein hormones which are produced in the pituitary or the placenta. It finds its use in many fertility related applications. Expression of bFSH in baculovirus/insect cell systems leads to a recombinant bFSH which has an unexpected high activity in a human FSH receptor assay and/or a bovine immature oocyte assay. The genes encoding the subunits of bFSH may be present on one baculovirus derived vector or on two or more vectors which are to be cotransfected.

Production of biologically active recombinant bovine follicle stimulation hormone (rec bFSH) in the baculovirus expression system

The invention provides a nucleotide sequence corresponding to a classical swine fever virus (CSFV) genome or a part or a mutant thereof, which comprises at least a part of the nucleotide sequence of the CSFV C-strain depicted in SEQ ID No. 1, or a complement or RNA equivalent of such nucleotide sequence, or which comprises a nucleotide sequence encoding at least the amino acid sequence 268-494 of the amino acid sequence depicted in SEQ ID No. 1, or a complement or RNA equivalent of such nucleotide sequence. Also provided is a pestivirus polypeptide corresponding to the amino acid sequence 690-1063 of SEQ ID No. 1 or part thereof, which contains a mutation in one of the epitopes within amino acid sequences 691-750 or 785-870, said mutation altering said epitope. Further provided is a method of determining the presence of a test substance capable of specifically binding with a binding site of a binding partner, in a sample, by means of competition of said test substance with a measurable amount of a reference substance capable of specifically binding with the same binding site of said binding partner, comprising: (1) contacting said sample with (a) said reference substance bound to a solid carrier, (b) the binding partner of said reference substance, said binding partner molecule containing at least two identical binding sites for said reference substance, and (c) said reference substance provided with a label; (2) measuring the degree of binding of said label to said carrier.

Growing community of inventors

Dronten, Netherlands

Robertus Jacobus Maria Moormann