Merck Patent Gmbh

Merck Patent Gesellschaft

Robertus Hendriks

Dieter Lubda

Klaus Unger

Uwe Michelsen

Sven Andrecht

Maria Wehsling

Karl Holschuh

Achim Schwämmle

Anette Boerner

Cora Rueter

Zöfre Bayram-Hahn

Afsaneh Abdolzade-Bavil

Andrea Lantos

Jonas Anders

The present invention relates to inorganic monolithic mouldings whose surface is coated with physi- or chemisorbed organic polymers, and to processes for the production of materials of this type. The materials according to the invention are highly suitable as sorbents for chromatography, in particular for high pressure liquid chromatography of biological materials.

Inorganic monolithic mouldings coated with organic polymers

The invention relates to a method and a kit for the isolation of phosphorylated peptides from complex mixtures using support materials having chelate ligands based on silica and alkaline elution buffers. In preferred embodiments, the method according to the invention enables the isolation of phosphopeptides from complex sample solutions with a reproducibly high yield and at the same time with high purity and it allows efficient ionisation and detection of the isolated phosphopeptides both by MALDI-TOF and also by ESI mass spectrometry without additional requisite method steps, such as, for example, chromatographic desalination of the sample.

Method and kit for the isolation of phosphorylated peptides

The present invention relates to a method and a kit for the sequential extraction of proteins for the production of partial proteomes from a complete proteome. In particular, the method according to the invention, besides other partial proteomes, gives a partial proteome of proteins from the cell nucleus interior separated from proteins of the cytoskeleton and of the nuclear matrix.

Method for extracting proteins

The invention relates to a method for removing endotoxins from nucleic acids. The endotoxins are removed by pre-incubating the nucleic acids in a salt-free detergent solution and subsequent anion exchange chromatography on a tentacle anion exchanger.

Method for removing endotoxins

The invention provides a method for isolating and purifying nucleic acids from liquid samples. The method comprises using a solid carrier which is essentially made of oxide inorganic materials containing hydroxyl groups (e.g., silica gel or hydroxylapatite). The nucleic acids are bound to the carrier material in the acidic pH range while they are eluted in the alkaline pH range.

Method for isolating and purifying nucleic acids

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Heidelberg, Germany

Robertus Hendriks