Dsm IP Assets B.v.

Robertus Antonius Mijndert Van Der Hoeven

Luppo Edens

Viktor Marius Boer

Peter Philip Lankhorst

Petrus Jacobus Theodorus Dekker

Igor Galaev

Silvia Gosiewska

Martina Beishuizen

Andre Leonardus De Roos

Dick Schipper

Noel Nicolaas Maria Elisabeth Van Peij

Marcus Hans

Olaf Leonardus Schouten

Linda De Lange

Veronique Delest

Melissa Harvey

The present invention relates to a steviol glycoside having the formula of (I) 

Steviol glycosides

The present invention relates to a method for the production of a compound of interest by microbial fermentation, wherein the microbial host cell used has been modified in its genome such that it results in a deficiency in the production of at least one non-ribosomal peptide synthase. The present invention further relates to a microbial host cell that has been modified in its genome such that it results in a deficiency in the production of at least one non-ribosomal peptide synthase. The invention further relates to a compound of interest.

Recombinant host cell with deficiency in non-ribosomal peptide synthase production

The present invention relates to a process for the proteolytic hydrolysis of a peptide or a polypeptide, said peptide or polypeptide comprising 4 to 40, preferably 5 to 35, amino acid residues and said peptide or polypeptide is not hydrolysable by subtilisin whereby said peptide or polypeptide is hydrolysed by a proline specific endo protease at a pH of 6.5 or lower, preferably 5.5 or lower and more preferably 5.0 or lower to hydrolyse said peptide or polypeptide.

Use of proline specific endoproteases to hydrolyse peptides and proteins

A method of enzymatically producing a protein hydrolysate from a protein substrate is described, wherein a proline-specific endoprotease or a composition containing a proline-specific endoprotease and optionally a subtilisin or a metallo endoprotease, and other enzymes such as carboxypeptidases, is used to produce a protein hydrolysate enriched in peptide fragments having a carboxy terminal proline residue. Such protein hydrolysates may be used as such or to reduce bitterness in foods nutritionally supplemented by protein hydrolysates, as well as to produce hydrolysate containing foodstuffs having low antigenicity.

Protein hydrolysates enriched in peptides having a carboxy terminal proline residue

The present invention provides An isolated polypeptide which has proline specific endoprotease activity, selected from the group consisting of:(a) a polypeptide which has an amino acid sequence which has at least 40% amino acid sequence identity with amino acids 1 to 526 of SEQ ID NO:2 or a fragment thereof;(b) a polypeptide which is encoded by a polynucleotide which hybridizes under low stringency conditions with (i) the nucleic acid sequence of SEQ ID NO:1 or a fragment thereof which is at least 80% or 90% identical over 60, preferably over 100 nucleotides, more preferably at least 90% identical over 200 nucleotides, or (ii) a nucleic acid sequence complementary to the nucleic acid sequence of SEQ ID NO:1.

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Katwijk, Netherlands

Robertus Antonius Mijndert Van Der Hoeven