Illumina Cambridge Limited

Illumina Corporation

Roberto Rigatti

Jonathan Mark Boutell

Niall Anthony Gormley

Geoffrey Paul Smith

Tobias William Barr Ost

David Bentley

Colin Lloyd Barnes

Graham John Worsley

Min-Jui Richard Shen

Shujun Luo

Vincent Peter Smith

John Milton

Xiaolin Wu

Anthony Romieu

Andrea Sabot

Gerardo Turcatti

David James Earnshaw

Kevin L Gunderson

Xiaohai Liu

Jason Richard Betley

Eric Hans Vermaas

Isabelle Marie Julia Rasolonjatovo

Boyan Boyanov

Matthew William Kellinger

Maria Candelaria Rogert Bacigalupo

Jingwei Bai

Shankar Balasubramanian

John M Beierle

Klaus Maisinger

Isabelle Rasolonjatovo

Allen E Eckhardt

Helen Rachel Bignell

Raquel Maria Sanches-Kuiper

Susan Shanahan

Frank J Steemers

Mostafa Ronaghi

Jeffrey S Fisher

Kay Klausing

John Moore

Kevin Hall

Dirk Evers

Raquel Maria Sanches

Avgousta Ioannou

Tobias Ost

Sarah Fashena

Sarah J Fashena

Liu Xiaohai

Isabelle Marie Rasolonjatovo

Epameinondas Fritzilas

Niall Gormley

Vincent Smith

Isabelle Marie J Rasolonjatovo

The present disclosure relates to the field of molecular biology and more specifically to microarrays and methods. In particular, the present disclosure relates to a method for amplifying a nucleic acid on a substrate comprising at least one well, the method includes: depositing a plurality of first capture primer pairs and a plurality of second capture primer pairs in a layer at least partially covering the inner well surface, wherein the primer density of first capture primer pairs is higher than the primer density of second primer pairs; contacting a sample comprising a plurality of target polynucleotides with the substrate under conditions sufficient for a single target polynucleotide per well to hybridize with a capture primer of the second capture primer pair; and performing a kinetic exclusion assay (KEA) to produce a monoclonal population of amplicons from the single target polynucleotide.

Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid

Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first portion of each polynucleotide molecule is attached to a first location of the surface and a second portion of each polynucleotide molecule is attached to a second location of the surface, the relative proximity of the first and second locations being correlated with the probability that the first and second portions are paired, separating the first and second portions of the polynucleotide molecules on the surface, determining the sequences of the first and second portions of the polynucleotide molecules and comparing the relative proximities and the sequences to determine which first and second portions are paired and to determine the sequence of the target polynucleotide molecules.

Methods for sequencing polynucleotides

The present invention relates to a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5' ends and at their 3′ ends.

Method of preparing libraries of template polynucleotides

The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.

Method for selecting and amplifying polynucleotides

The present invention relates to methods of imaging template hybridisation for estimating cluster numbers prior to solid phase amplification and sequencing. More particularly, an initial round of imaging is carried out at the single molecule template hybridisation stage which allows a general estimation of cluster numbers prior to clusters being formed. Amplification of the signal allows single molecule imaging to be carried out using standard sequencing imaging apparatus.

Methods for estimating cluster numbers

The present disclosure relates to the field of molecular biology and more specifically to microarrays and methods, including methods for modifying immobilized capture primers comprising: a) contacting a substrate comprising a plurality of immobilized capture primers with a plurality of template nucleic acids under conditions sufficient for hybridization to produce one or more immobilized template nucleic acids, and b) extending one or more immobilized capture primers to produce one or more immobilized extension products complementary to the one or more template nucleic acid.

Embodiments of the present invention relate to analyzing components of a cell. In some embodiments, the present invention relate to analyzing components of a single cell. In some embodiments, the methods and compositions relate to sequencing nucleic acids. In some embodiments, the methods and compositions relate to identifying and/or quantitating nucleic acid, proteins, organelles, and/or cellular metabolites.

Methods and compositions for analyzing cellular components

The present disclosure provides methods and systems for determining and/or characterizing one or more haplotypes and/or phasing of haplotypes in a nucleic acid sample. In particular, the disclosure provides methods for determining a haplotype and/or phasing of haplotypes in a nucleic acid sample by incorporating synthetic polymorphisms into fragments of a nucleic acid sample and utilizing the synthetic polymorphisms in determining one or more haplotypes and/or phasing of haplotypes.

Methods and systems for determining haplotypes and phasing of haplotypes

Embodiments provided herein relate to methods and compositions for next generation sequencing. Some embodiments include the preparation of a template library from a target nucleic acid in contact with a surface, and sequencing the library on the surface.

Methods and compositions for preparing sequencing libraries

The present invention relates to a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5' ends and at their 3&#x2032; ends.

The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.

Method for sequencing a polynucleotide template

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Nr Saffron Walden, United Kingdom

Roberto Rigatti