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Eni-ente Nazionale Idrocarburi

E.n.i. Nazionale Idrocarburi

E.n.i. Ente Nozionale Idrocarburi

Roberto Olivieri

Ludwig Degen

Eugenio Fascetti

Felice Centini

Giancarlo Eletti Bianchi

Walter Marconi

Andrea Robertiello

Pierluigi Ciuffolotti

Nadia Cimini

Paolo Branduzzi

Aurelio Viglia

Leonello Angelini

Nicola Oddo

Paolo Pansolli

The process of the invention is useful for the preparation of L-.alpha.-aminoacids and is based on the use of an N-carbamyl-.alpha.-aminoacid compound which is contacted with a E.C.3.5.2.2, a dihydropyrimidine hydrolase, enzyme to form a D-hydantoin. The D-hydantoin is hydrolyzed and racemized to form the D-N-carbamyl-.alpha.-aminoacid and the L-N-carbonyl-.alpha.-aminoacid. The L-N-carbamyl-.alpha.-aminoacid is thereafter transformed into the corresponding L-.alpha.-aminoacid.

Process for the preparation of L-.alpha.-aminoacids

This invention relates to a process for the preparation of D(-) mandelic acid by the acid hydrolysis of the corresponding carbamate in an aqueous environment at a temperature of between 40.degree. and 100.degree. C. and at a pH of between 1 and 3.5. The invention also relates to a process for the enzymatic hydrolysis of racemic 5-substituted 2,4-oxazolidinediones to give only one of the two possible optical isomers, i.e., the D-carbamyl-.alpha.-hydroxy acid. The free D-.alpha.-hydroxy acid can be obtained from the optically active carbamyl derivative by simple hydrolysis. Of particular interest is the case in which the D-.alpha.-hydroxy acid is D(-)mandelic acid. The enzymatic activity required for preparing the carbamyl derivative of D(-)mandelic acid has been found both in homogenised veal liver and in a series of microorganisms, including Agrobacterium radiobacter, Bacillus brevis, Bacillus stearothermophilus, Pseudomonas sp., Pseudomonas desmolytica, Pseudomonas fluorescens, Pseudomonas putida.

Process for producing D(-)mandelic acid

The enzyme alpha-galactosidase is prepared from a constitutive mutant of the strain Saccharomyces cerevisiae NRRL-Y-12057, said mutant being marked by the identification number NRRL-Y-12533.

Process for the production of alpha-galactosidase and uses of the enzyme

Compositions are disclosed, which are adapted to depollute fresh and sea water bodies from crude oil and petroleum product pollution by microbial action. The growth of micro-organisms capable of metabolizing hydrocarbons is exalted by certain combination of nutrients, such as lecithin as a phosphorus source, hydantoins, amides allophanates, polyamines, acyl-ureas and esters of the hydantoic and allantoic acids as the nitrogen sources. Ureido-derivatives of amides are also contemplated as additional nutrients.

Compositions for depolluting fresh water and salt water bodies

It has been found that highly active uricase can be produced by fermentation from the microorganism Micrococcus roseus NRRL B 12196. The optimum activity of this microorganism is close to the pH values of the physiological liquors. The uricase so produced can be exploited in the clinical diagnostics for determining uric acid.

Uricase production method

This invention relates to a process for preparing carbamyl derivatives of .alpha.-hydroxy acids by the hydrolysis of 5-substituted 2,4-oxazolidinediones. The carbamyl derivatives can be further hydrolyzed to .alpha.-hydroxy acids. The invention also relates to a process for the enzymatic hydrolysis of racemic 5-substituted 2,4-oxazolidinediones to give only one of the two possible optical isomers, i.e. the D-carbamyl-.alpha.-hydroxy acid. The free D-.alpha.-hydroxy acid can be obtained from the optically active carbamyl derivative by simple hydrolysis. Of particular interest is the case in which the D-.alpha.-hydroxy acid is D(-)mandelic acid. The enzymatic activity required for preparing the carbamyl derivative of D(-)mandelic acid has been found both in homogenized veal liver and in a series of microorganisms, including Agrobacterium radiobacter, Bacillus brevis, Bacillus stearothermophilus, Pseudomonas sp., Pseudomonas desmolytica, Pseudomonas fluorescens, Pseudomonas putida.

Process for preparing carbamyl derivatives of .alpha.-hydroxy acids and

A method is disclosed for producing D-aminoacids from racemic mixtures of N-carbamoyl derivatives of such acids or from the corresponding hydantoins wherein the racemic compounds are subjected to the enzymic and microbiological action of an enzymic complex prepared from microorganisms of the Agrobacterium genus, more particularly the NRRL B 11291 strain.

Enzymic microbiological process for producing optically active

A method is disclosed for the production of fructose and syrups containing fructose and glucose, comprising the step of contacting a solution of glucose with a micro-organism of the genus Streptomyces sp. and more particularly of the strains NRRL 11.120 and NRRL 11.121, as designated by the Northern Regional Research Center, U.S. Department of Agriculture, Peoria, Ill.

Production of fructose and fructose-base syrups and means for carrying

A method and a few compositions are disclosed, which are adapted to depollute fresh and sea water bodies from crude oil and petroleum product pollution by microbial action. The growth of micro-organisms capable of metabolizing hydrocarbons is exalted by certain combination of nutrients, such as lecithin as a phosphorus source, hydantoins, amides allophanates, polyamines, acyl-ureas and esters of the hydantoic and allantoic acids as the nitrogen sources. Ureido-derivatives of amides are also contemplated as additional nutrients.

Method for depolluting fresh water and salt water bodies from crude oil,

L-malic acid is prepared from fumaric acid through a microbiological fermentation process employing a microbial strain of the Paracolobactrum genus having the number 743, 745 or 746.

Growing community of inventors

Mentana, Italy

Roberto Olivieri