Creagri, Inc.

Other

Protelica, Inc.

Pfizer Corporation

Harvard College

Elan Pharmaceuticals, Inc.

Neurex Corporation

Symbiotech Incorporated

Oliphenol, LLC

Agroils Technologies Srl

Creatrone, Inc.

Bioren, Inc.

Medical Science Partners

Protelix, Inc.

Roberto Crea

Guido Cappuccilli

Randy Shen

Craig A Hokanson

Peter B Kirk

David R Liston

Edmund C Lin

Bryan L Ray

Catherine M Bitler

Luciano Caglioti

Stephen S Bowersox

Susan D Demo

William A Horne

Mei Zhou

Arvind Rajpal

Toshihiko Takeuchi

Ramesh Bhatt

Tokuko Saito Wiedemann

Nurten Beyaz-Kavuncu

Paolo Pontoniere

Jun Yoon

Emanuela Zacco

Fujio Numano

Takeuchi Toshihilo

Tokuko S Wiedemann

Nurten Bayez

Guido Cappucilli

Provided herein are chimeric antigen receptors (CARs) for binding with a target antigen, comprising at least one antigen specific targeting region comprising a fibronectin type 3 (FN3) domain polypeptide. Provided herein are multispecific chimeric antigen receptors for binding with two or more target antigens, comprising at least two antigen specific targeting regions comprising a fibronectin type 3 domain polypeptide. Also provided herein are compositions and methods of treatment relating to the use of subject CARs of the invention.

Fibronectin binding domain chimeric antigen receptors and methods of use thereof

Use of an olive oil milling by-product (Alperujo or Olive Pomace) for the co-processing and co-extraction of natural compounds from medicinal and aromatic plants including cannabis, fruits and agricultural waste, algae and other microorganisms. Separation and stabilization of fractions containing either hydrophilic or liposoluble compounds.

Olive oil use

Universal antibody libraries are described which are synthetic and derived from expressed human antibody sequences selected accordingly to certain criteria, for example, that the sequences are derived from naturally-occurring antibodies expressed in response to a certain antigen class (e.g., small molecule, polysaccharide, peptide, or protein) and having CDR regions engineered for optimal diversity. Methods for making and screening such libraries for isolating therapeutics suitable for treating disease are also disclosed.

Universal antibody libraries

Use of an olive oil milling by-product (Alperujo or Olive Pomace) for the co-processing and co-extraction of natural compounds from medicinal and aromatic plants, fruits and agricultural waste, algae and other microorganisms. Separation and stabilization of fractions containing either hydrophilic or liposoluble compounds. The plants may include

Methods are disclosed to create healthier palm oil and new food additives derived from products of the processing offruit that were hitherto fore discarded as waste. The pressing product offruit and the effluent created is treated with acidified antioxidants from olive vegetation containing hydroxytyrosol and a mixture of other polyphenols. The treated POME, palm oil and palm juice are stabilized against oxidation and their inherently nutritive components may now be harvested instead of being thrown away.

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Compositions obtained from vegetation water from olives and methods for treating patients suffering from an inflammatory skin disease with the compositions are described. Included are hydroxytyrosol rich compounds obtained from olive vegetation water.

Vegetation water composition for treatment of inflammatory skin conditions

A process for preparing a food or feed composition fromis disclosed. The method involves adding an acidified aqueous solution tocomponents, to a final pH of between 1 and 5, incubating the acidified mixture for a period for a period of at least 1 hour, and centrifuging the incubated mixture to separate the mixture into three physically distinct fractions: (i) a light, upper fraction containing oil, (ii) an aqueous fraction containing soluble acid-extracted components and breakdown products, and (iii) a substantially detoxified solid cake which forms or is used in forming the food or feed composition. The acidified aqueous solution added may be acidified olive vegetation water having a ratio of hydroxytyrosol to oleuropein of between 5:1 to 100:1. Also disclosed are a food or feed composition, and oil and aqueous fractions formed by the method.

Jatropha curcas processing method and products

Fibronectin Type III (FN3) polypeptide libraries are described, along with their use in identifying fibronectin-type binding peptides having high binding affinities, e.g., greater than 300 nM, for VEGFR2 or Axl proteins.

Universal fibronectin type III binding-domain libraries

A composition comprising individual single-stranded oligonucleotides capable of self assembling to form a pair of complementary, substantially contiguous single strands of a double-stranded nucleic acid filament, and a method for forming such filaments are disclosed.

Self-assembling oligonucleotides and methods

A method of mutagenesis by which a predetermined amino acid is introduced into each and every position of a selected set of positions in a preselected region (or several different regions) of a polypeptide to produce a library of polypeptide analogs is disclosed. The method is based on the premise that certain amino acids play a crucial role in the structure and function of proteins and thus is capable of identifying and distinguishing functional amino acid residues ('hot spots') from non-functional amino acids residues ('cold spots') within a polypeptide or portion thereof. Libraries can be generated which contain only desired polypeptide analogs and are of reasonable size for screening. The libraries can be used to study the role of specific amino acids in polypeptide structure and function and to develop new or improved polypeptides such as antibodies, antibody fragments, single chain antibodies, enzymes, and ligands.

Look-through mutagenesis for developing altered polypeptides with enhanced properties

Methods and compositions directed to improved universal antibody libraries that rationally exploit human diversity information contained within reference antibody libraries, such as universal antibody libraries, are disclosed. The disclosed processes involve use of a query CDR sequence to guide incorporation of human antibody diversity present within the reference library into cohort libraries of the invention. Methods for making and screening such cohort libraries for isolating therapeutics suitable for treating disease are also disclosed.

Antibody ultrahumanization by predicted mature CDR blasting and cohort library generation and screening

Walk-through mutagenesis and natural-variant combinatorial fibronectin Type III (FN3) polypeptide libraries are described, along with their method of construction and use. Also disclosed are a number of high binding affinity polypeptides selected by screening the libraries against a variety of selected antigens.

Universal fibronectin Type III binding-domain libraries

The invention provides olive-derived hydroxytyrosol. According to one aspect of the invention, vegetation water is collected from olives. Acid is added to stabilize the vegetation water and prevent fermentation. The mixture is incubated to allow oleoeuropein to convert to hydroxytyrosol, and then fractionated to separate hydroxytyrosol from other components. The hydroxytyrosol is useful as a natural anti-bacterial, anti-viral and fungicidal product for agricultural and pest control applications. In addition, it is useful as a therapeutic and anti-oxidant for a variety of health purposes.

Method of obtaining a hydroxytyrosol-rich composition from vegetation water

Growing community of inventors

San Mateo, CA, United States of America

Roberto Crea