Syva Company

Roberta D Ernst

Richard K Leute

Judith I Blakemore

An enzyme-polyidothyronine conjugate for use in determining polyiodothyronine by immunoassay is prepared containing an enzyme which is reversibly deactivated by polyiodothyronine and is reactivated by an antibody binding to the polyiodothyronine of the conjugate. Preferred enzymes are malate dehydrogenase and triose phosphate isomerase.

Enzyme-bound-polyidothyronine

Method and enzyme conjugates are provided for determining physiological amounts of polyiodothyronine in physiological fluid, particularly serum. A homogeneous enzyme immunoassay is provided employing an enzyme-bound-polyiodothyronine having less than about 50 percent of the original enzyme activity, whose activity increases upon binding with a receptor. The enzyme-bound-polyiodothyronine, the unknown suspected to containing a polyiodothyronine, and receptor for the polyiodothyronine are combined in an aqueous medium containing substrate for said enzyme and the enzymatic activity of the solution determined. By comparing the result obtained with results obtained from standards having known amounts of polyiodothyronine, the amount of polyiodothyroninein the unknown can be determined. The enzyme-bound-polyiodothyronine employs an enzyme which is reversibly deactivated by polyiodothyronine, so as to be reactived by receptor binding to the polyiodothyronine. Particular enzymes include malate dehydrogenase and triose phosphate isomerase.

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Mountain View, CA, United States of America

Roberta D Ernst