Dade Behring Marburg Gmbh

Behringwerke Aktiengesellschaft

Robert Ziegelmaier

Werner Stuber

Stefan Knapp

Leszek Wieczorek

Hans K&uuml;pper

Hans Kupper

An immunochemical method for the determination of antibodies which are specific for an antigen and are of one of the immunoglobulin classes: A, M, D or E in a fluid, with this fluid being contacted with a solid phase to which the antibodies against this immunoglobulin class, or a fragment of an antibody of this type, are bound, which results in the immunoglobulin of this class being bound to this solid phase, and this solid phase being contacted with the antigen, which carries a labeling means where appropriate, and with a labeled antibody or a labeled fragment of an antibody against the antigen if the antigen is unlabeled and determination, from the amount of labeling means which is bound to the solid phase, of the amount of these antibodies which are specific for an antigen and are one of the immunoglobulin classes, which comprises the solid phase being simultaneously in contact with the fluid containing the antibody which is to be determined and with the antigen, which is labeled where appropriate, there being addition of a substance which prevents immunoglobulin G binding to the solid phase and, where appropriate, antigen binding to immunoglobulin G, and an agent suitable for this purpose, are described.

One-step immunoassay for the determination of antigen-specific antibodies of one of the immunoglobulin classes A, M, D, or E, and an agent suitable for this purpose

The present invention relates to the identification of antigens and the preparation thereof by genetic engineering. A cDNA expression gene bank of this parasite was prepared. Recombinant clones which are of diagnostic interest were identified using a high-titer rabbit anti- serum, and isolated.

Toxoplasma gondii antigens, the preparation thereof and the use thereof

The present invention relates to the identification of toxoplasma gondii antigens and the preparation thereof by genetic engineering. A cDNA expression gene bank of this parasite was prepared. Recombinant clones which are of diagnostic interest were identified using a high-titer rabbit anti-Toxoplasma gondii serum, and isolated.

The invention relates to peptides for the immunochemical determination of human cytomegalovirus (HCMV)-specific antibodies and HCMV antigens, and to agents suitable for this method and to the use thereof.

HCMV-specific peptides, agents therefor and the use thereof

The present invention relates to the identification of Toxoplasma gondii antigens and the preparation thereof by genetic engineering. A cDNA expression gene bank of this parasite was prepared. Recombinant clones which are of diagnostic interest were identified using a high-titer rabbit anti-Toxoplasma gondii serum, and isolated.

The invention relates to a DNA sequence which encodes three specific human cytomegalovirus (HCMV) peptides of the pp150 protein. These peptides are shown to have novel immunogenic properties. The DNA sequence is also used in a method of hybridization for the detection of fully complementary sequences.

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Marburg, Germany

Robert Ziegelmaier